5'-Nucleotidase (NT5E) Active Protein | NT5E active protein

Recombinant Human 5'-Nucleotidase (NT5E), Partial

Cat. Num. AAA7115104

• Gene Names: NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 5'-Nucleotidase (NT5E); Recombinant Human 5'-Nucleotidase (NT5E); Partial; 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE; NT5E active protein

• Host: Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: 0.2 mum Filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5
• Sequence Positions: 27-547aa; Partial
• Sequence: WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK
• Sequence Length: 574

• Species: Human
• Tag: C-terminal 6xHis-tagged
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Biological Activity: Specific activity as determined by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP) is greater than 15000 pmol/min/ug. The orthophosphate product is measured by a malachite green phosphate detection kit.
• Subcellular Location: Cell Membrane, Lipid-Anchor, GPI-Anchor
• Protein Families: 5'-Nucleotidase Family
• Classification: Drug Target
• Subdivision: CD Antigen
• Preparation and Storage: Store at -20 degree C. For extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for NT5E active protein

Relevance: CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Product Categories/Family for NT5E active protein

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 112825
• NCBI GeneID: 4907
• UniProt Accession #: P21589
• Molecular Weight: 58.76 kDa
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5'-nucleotidase ecto
• NCBI Official Symbol: NT5E
• NCBI Official Synonym Symbols: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: NT5E
• UniProt Synonym Gene Names: NT5; NTE; 5'-NT
• UniProt Entry Name: 5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries