Rabbit anti-Human BARD1 Polyclonal Antibody | anti-BARD1 antibody

BARD1 Antibody

Cat. Num. AAA7114454

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >95%; Protein G Purified
• Synonyms: BARD1; Polyclonal Antibody; BARD1 Antibody; BRCA1-associated RING domain protein 1; RING-type E3 ubiquitin transferase BARD1; anti-BARD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%; Protein G Purified
• Form/Format: Liquid
• Sequence Length: 777

• Applicable Applications for anti-BARD1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:5000
• Immunogen Species: Human
• Immunogen: Recombinant Human BRCA1-associated RING domain protein 1 protein (428-777AA)
• Conjugate: Non-Conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate All lanes: BARD1 antibody at 3.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 87, 85, 76, 15 kDaObserved band size: 87 kDa)

Product Categories/Family for anti-BARD1 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 157266328
• NCBI GeneID: 580
• NCBI Accession #: NP_000456.2
• NCBI GenBank Nucleotide #: NM_000465.4
• UniProt Accession #: Q99728; O43574; Q53SS5; F6MDH7; F6MDH8; F6MDH9
• Molecular Weight: 84,574 Da
• NCBI Official Full Name: BRCA1-associated RING domain protein 1 isoform 1
• NCBI Official Synonym Full Names: BRCA1 associated RING domain 1
• NCBI Official Symbol: BARD1
• NCBI Protein Information: BRCA1-associated RING domain protein 1
• UniProt Protein Name: BRCA1-associated RING domain protein 1
• Protein Family: BRCA1-associated RING domain protein
• UniProt Gene Name: BARD1
• UniProt Synonym Gene Names: BARD-1

NCBI Description

This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.