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Immunohistochemistry (IHC) (IHC image of CSB-PA688972LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Rabbit anti-Human TAF7L Polyclonal Antibody | anti-TAF7L antibody

TAF7L Antibody

Gene Names
TAF7L; CT40; TAF2Q
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunofluorescence
Purity
>95%
Protein G Purified
Synonyms
TAF7L; Polyclonal Antibody; TAF7L Antibody; Transcription initiation factor TFIID subunit 7-like; Cancer/testis antigen 40; CT40; RNA polymerase II TBP-associated factor subunit Q; TATA box-binding protein-associated factor 50 kDa; Transcription initiation factor TFIID 50 kDa subunit; TAF2Q; anti-TAF7L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Form/Format
Liquid
Sequence Length
376
Applicable Applications for anti-TAF7L antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 1:200-1:500
IF: 1:50-1:200
Immunogen Species
Human
Immunogen
Recombinant Human Transcription initiation factor TFIID subunit 7-like protein (1-98AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(IHC image of CSB-PA688972LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of CSB-PA688972LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescence staining of HepG2 cells with CSB-PA688972LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of HepG2 cells with CSB-PA688972LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Product Categories/Family for anti-TAF7L antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,570 Da
NCBI Official Full Name
transcription initiation factor TFIID subunit 7-like isoform 2
NCBI Official Synonym Full Names
TATA-box binding protein associated factor 7 like
NCBI Official Symbol
TAF7L
NCBI Official Synonym Symbols
CT40; TAF2Q
NCBI Protein Information
transcription initiation factor TFIID subunit 7-like
UniProt Protein Name
Transcription initiation factor TFIID subunit 7-like
UniProt Gene Name
TAF7L
UniProt Synonym Gene Names
TAF2Q; CT40

NCBI Description

This gene is similar to a mouse gene that encodes a TATA box binding protein-associated factor, and shows testis-specific expression. The encoded protein could be a spermatogenesis-specific component of the DNA-binding general transcription factor complex TFIID. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

Probably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis ().

Research Articles on TAF7L

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Product Notes

The TAF7L taf7l (Catalog #AAA7113982) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TAF7L Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TAF7L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:200-1:500 IF: 1:50-1:200. Researchers should empirically determine the suitability of the TAF7L taf7l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAF7L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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