Immunohistochemistry (IHC)
(IHC image of MBS7107087 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. )
Rabbit anti-Human SIN3B Polyclonal Antibody | anti-SIN3B antibody
SIN3B Antibody
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
SIN3B; Polyclonal Antibody; SIN3B Antibody; Paired amphipathic helix protein Sin3b; Histone deacetylase complex subunit Sin3b; Transcriptional corepressor Sin3b; anti-SIN3B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
1130
Applicable Applications for anti-SIN3B antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 1:200-1:500
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Paired amphipathic helix protein Sin3b protein (705-769AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Immunohistochemistry (IHC)
(IHC image of MBS7107087 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. )
Immunohistochemistry (IHC)
(IHC image of MBS7107087 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. )
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7107087 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7107087 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-SIN3B antibody
Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression.
Product Categories/Family for anti-SIN3B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,507 Da
NCBI Official Full Name
paired amphipathic helix protein Sin3b isoform 2
NCBI Official Synonym Full Names
SIN3 transcription regulator family member B
NCBI Official Symbol
SIN3B
NCBI Protein Information
paired amphipathic helix protein Sin3b
UniProt Protein Name
Paired amphipathic helix protein Sin3b
Protein Family
UniProt Gene Name
SIN3B
Uniprot Description
Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression.
Research Articles on SIN3B
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Product Notes
The SIN3B sin3b (Catalog #AAA7107087) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIN3B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIN3B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:200-1:500 IF: 1:50-1:200. Researchers should empirically determine the suitability of the SIN3B sin3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIN3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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