Human melanoma antigen family A, 3 ELISA Kit | MAGEA3 elisa kit

Human melanoma-associated antigen 3, MAGE-3 ELISA Kit

Cat. Num. AAA705585

• Gene Names: MAGEA3; HIP8; HYPD; CT1.3; MAGE3; MAGEA6
• Reactivity: Human
• Synonyms: melanoma antigen family A; 3; Human melanoma-associated antigen 3; MAGE-3 ELISA Kit; Human melanoma-associated antigen 3 (MAGE-3) ELISA Kit; CT1.3; HIP8; HYPD; MAGE3; MAGEA6; MGC14613; MAGE-3 antigen; antigen MZ2-D; cancer/testis antigen family 1; member 3; melanoma-associated antigen 3; MAGEA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human MAGE-3. No significant cross-reactivity or interference between human MAGE-3 and analogues was observed.
• Sequence Length: 314

• Samples: Serum, plasma, tissue homogenates, urine
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625 ng/ml-40 ng/ml
• Sensitivity: < 0.156 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAGEA3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAGE-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAGE-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAGE-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAGE-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4885467
• NCBI GeneID: 4102
• NCBI Accession #: NP_005353.1
• NCBI GenBank Nucleotide #: NM_005362.3
• UniProt Accession #: P43357; Q6FHI6
• Molecular Weight: 34,747 Da
• NCBI Official Full Name: melanoma-associated antigen 3
• NCBI Official Synonym Full Names: melanoma antigen family A, 3
• NCBI Official Symbol: MAGEA3
• NCBI Official Synonym Symbols: HIP8; HYPD; CT1.3; MAGE3; MAGEA6
• NCBI Protein Information: melanoma-associated antigen 3; antigen MZ2-D; MAGE-3 antigen; cancer/testis antigen 1.3; cancer/testis antigen family 1, member 3
• UniProt Protein Name: Melanoma-associated antigen 3
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEA3
• UniProt Synonym Gene Names: MAGE3; CT1.3
• UniProt Entry Name: MAGA3_HUMAN

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.1 PublicationManual assertion based on experiment in:Ref.9