Rat oxidized low density lipoprotein (lectin-like) receptor 1 ELISA Kit | OLR1 elisa kit

Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1, sLOX-1 ELISA Kit

Cat. Num. AAA705578

• Gene Names: OLR1; LOX1; LOX-1
• Reactivity: Rat
• Synonyms: oxidized low density lipoprotein (lectin-like) receptor 1; Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1; sLOX-1 ELISA Kit; Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1 (sLOX-1) ELISA Kit; CLEC8A; LOX1; SCARE1; lectin-type oxidized LDL receptor 1; oxidised low density lipoprotein (lectin-like) receptor 1; scavenger receptor class E; member 1; OLR1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat sLOX-1. No significant cross-reactivity or interference between rat sLOX-1 and analogues was observed.
• Sequence Length: 278

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OLR1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sLOX-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sLOX-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sLOX-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sLOX-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 130493907
• NCBI GeneID: 100009322
• NCBI Accession #: NP_001076102.1
• NCBI GenBank Nucleotide #: NM_001082633.1
• UniProt Accession #: Q9XTA8
• Molecular Weight: 31,159 Da
• NCBI Official Full Name: oxidized low-density lipoprotein receptor 1
• NCBI Official Symbol: OLR1
• NCBI Official Synonym Symbols: LOX1; LOX-1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• Protein Family: Oxidized low-density lipoprotein receptor
• UniProt Gene Name: OLR1
• UniProt Synonym Gene Names: LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1
• UniProt Entry Name: OLR1_RABIT

Uniprot Description

Function: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria