Rabbit anti-Human EPHB4 Polyclonal Antibody | anti-EPHB4 antibody

EPHB4 Antibody

Cat. Num. AAA7046157

• Gene Names: EPHB4; HTK; MYK1; TYRO11
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: EPHB4; Polyclonal Antibody; EPHB4 Antibody; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; Tyrosine-protein kinase TYRO11; HTK; MYK1; TYRO11; anti-EPHB4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 987

• Applicable Applications for anti-EPHB4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Ephrin type-B receptor 4 protein (300-540AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7285 / sc-7284-R / sc-130753 / sc-5536
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: EPHB4 antibody at 2.71ug/mlLane 1: A549 whole cell lysateLane 2: 293T whole cell lysateLane 3: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 109,56,34,46 kDaObserved band size: 108 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver tissue MBS7046157 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue MBS7046157 at dilution 1:100)

Related Product Information for anti-EPHB4 antibody

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 32528301
• NCBI GeneID: 2050
• NCBI Accession #: NP_004435.3
• NCBI GenBank Nucleotide #: NM_004444.4
• UniProt Accession #: P54760; Q7Z635; Q9BTA5; Q9BXP0; B5A970; B5A971; B5A972
• Molecular Weight: 45,195 Da
• NCBI Official Full Name: ephrin type-B receptor 4
• NCBI Official Synonym Full Names: EPH receptor B4
• NCBI Official Symbol: EPHB4
• NCBI Official Synonym Symbols: HTK; MYK1; TYRO11
• NCBI Protein Information: ephrin type-B receptor 4
• UniProt Protein Name: Ephrin type-B receptor 4
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB4
• UniProt Synonym Gene Names: HTK; MYK1; TYRO11
• UniProt Entry Name: EPHB4_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]

Uniprot Description

EphB4: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Protein type: Kinase, protein; Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: cytosol; extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: ephrin receptor activity; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: angiogenesis; cell adhesion; cell migration during sprouting angiogenesis; ephrin receptor signaling pathway; heart morphogenesis; protein amino acid autophosphorylation