Rabbit anti-Human, Mouse CRBN Polyclonal Antibody | anti-CRBN antibody

CRBN Antibody

Cat. Num. AAA7044878

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: CRBN; Polyclonal Antibody; CRBN Antibody; Protein cereblon; AD-006; anti-CRBN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 441

• Applicable Applications for anti-CRBN antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Protein cereblon protein (N-280AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: CRBN antibody at 5.28 ug/mlLane 1: Mouse liver tissueLane 2: A375 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 51 kDaObserved band size: 51 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS7044878 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7044878 at dilution of 1:100)

Related Product Information for anti-CRBN antibody

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.

NCBI and Uniprot Product Information

• NCBI GI #: 291045198
• NCBI GeneID: 51185
• NCBI Accession #: NP_001166953.1
• NCBI GenBank Nucleotide #: NM_001173482.1
• UniProt Accession #: Q96SW2; Q6AI62; Q6NVZ0; Q9UHW4; B2R6H4; C9IZA9; C9JAH6
• Molecular Weight: 50,475 Da
• NCBI Official Full Name: protein cereblon isoform 2
• UniProt Protein Name: Protein cereblon
• Protein Family: Protein cereblon
• UniProt Gene Name: CRBN
• UniProt Entry Name: CRBN_HUMAN

Uniprot Description

CRBN: Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A). MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs. Belongs to the CRBN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination

Disease: Mental Retardation, Autosomal Recessive 2