Human membrane-spanning 4-domains, subfamily A, member 1 ELISA Kit | MS4A1 elisa kit

Human B-lymphocyte antigen, CD20 ELISA Kit

Cat. Num. AAA704400

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Reactivity: Human
• Synonyms: membrane-spanning 4-domains; subfamily A; member 1; Human B-lymphocyte antigen; CD20 ELISA Kit; Human B-lymphocyte antigen (CD20) ELISA Kit; B1; Bp35; CD20; LEU-16; MGC3969; MS4A2; S7; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; MS4A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CD20. No significant cross-reactivity or interference between human CD20 and analogues was observed.
• Sequence Length: 297

• Samples: Serum, plasma and tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12 ng/ml -200 ng/ml
• Sensitivity: Typically less than 0.78 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MS4A1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD20 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

References

Genomic Profile of Fatigued Men Receiving Localized Radiation Therapy. Chao-Pin Hsiao, PhD, RN, Swarnalatha Y. Reddy, PhD, Mei-Kuang Chen, PhD, Leorey N. Saligan, PhD, RN, CRNP, FAAN (2015)

NCBI and Uniprot Product Information

• NCBI GI #: 23110987
• NCBI GeneID: 931
• NCBI Accession #: NP_068769.2
• NCBI GenBank Nucleotide #: NM_021950.3
• UniProt Accession #: P11836; P08984; Q13963; A6NMS4
• Molecular Weight: 33,077 Da
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane-spanning 4-domains, subfamily A, member 1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1
• UniProt Protein Name: B-lymphocyte antigen CD20
• Protein Family: B-lymphocyte antigen
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN

NCBI Description

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: This protein may be involved in the regulation of B-cell activation and proliferation.