Mouse matrix metallopeptidase 3 (stromelysin 1, progelatinase) ELISA Kit | MMP3 elisa kit

Mouse matrix metalloproteinase 3, MMP-3 ELISA Kit

Cat. Num. AAA702504

• Gene Names: Mmp3; SLN1; Str1; SLN-1; STR-1; Stmy1
• Reactivity: Mouse
• Synonyms: matrix metallopeptidase 3 (stromelysin 1; progelatinase); Mouse matrix metalloproteinase 3; MMP-3 ELISA Kit; MMP-3 ELISA kit; CHDS6; MGC126102; MGC126103; MGC126104; MMP-3; SL-1; STMY; STMY1; STR1; matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1; progelatinase) ; proteoglycanase; transin-1; MMP3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse MMP3. No significant cross-reactivity or interference between Mouse MMP3 and analogues was observed.
• Sequence Length: 479

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.78 ng/ml-50 ng/ml
• Sensitivity: 0.195 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Developmental Protein
• Protein Biological Process 3: Collagen degradation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6754714
• NCBI GeneID: 17392
• NCBI Accession #: NP_034939.1
• NCBI GenBank Nucleotide #: NM_010809.1
• UniProt Accession #: P28862
• Molecular Weight: 53,845 Da
• NCBI Official Full Name: stromelysin-1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 3
• NCBI Official Symbol: Mmp3
• NCBI Official Synonym Symbols: SLN1; Str1; SLN-1; STR-1; Stmy1
• NCBI Protein Information: stromelysin-1; SL-1; EMS-2; MMP-3; transin-1; progelatinase; stromelysin 1; matrix metalloproteinase 3; matrix metalloproteinase-3
• UniProt Protein Name: Stromelysin-1
• Protein Family: Stromelysin
• UniProt Gene Name: Mmp3
• UniProt Synonym Gene Names: SL-1; MMP-3
• UniProt Entry Name: MMP3_MOUSE

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that is involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded protein is activated by the removal of an N-temrinal activation peptide to generate a zinc-dependent endopeptidase with a broad range of substrates such as proteoglycans, laminin, fibronectin, elastin, and collagens. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Aug 2015]

Uniprot Description

Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Catalytic activity: Preferential cleavage where P1', P2' and P3' are hydrophobic residues.

Cofactor: Binds 4 calcium ions per subunit