Mouse anti-Mullerian hormone ELISA Kit | AMH elisa kit

Mouse anti-Mullerian hormone, AMH ELISA Kit

Cat. Num. AAA701401

• Gene Names: Amh; MIS
• Reactivity: Mouse
• Synonyms: anti-Mullerian hormone; Mouse anti-Mullerian hormone; AMH ELISA Kit; Mouse anti-Mullerian hormone (AMH) ELISA kit; MIF; MIS; Mullerian inhibiting factor; Mullerian inhibiting substance; AMH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse AMH. No significant cross-reactivity or interference between mouse AMH and analogues was observed.
• Sequence Length: 555

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 0.38 ng/ml -150 ng/ml.
• Sensitivity: The minimum detectable dose of mouse AMH is typically less than 0.38 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse AMH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AMH elisa kit

Principle of the assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for AMH and Horseradish Peroxidase (HRP) conjugated AMH. The competitive inhibition reaction is launched between with HRP labeled AMH and unlabeled AMH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of AMH in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 127110
• NCBI GeneID: 11705
• UniProt Accession #: P27106
• Molecular Weight: 59,778 Da
• NCBI Official Full Name: Muellerian-inhibiting factor
• NCBI Official Synonym Full Names: anti-Mullerian hormone
• NCBI Official Symbol: Amh
• NCBI Official Synonym Symbols: MIS
• NCBI Protein Information: muellerian-inhibiting factor; anti-Muellerian hormone; Mullerian inhibiting substance; muellerian-inhibiting substance
• UniProt Protein Name: Muellerian-inhibiting factor
• Protein Family: Muellerian-inhibiting factor
• UniProt Gene Name: Amh
• UniProt Synonym Gene Names: AMH; MIS
• UniProt Entry Name: MIS_MOUSE

NCBI Description

This gene is a member of the transforming growth factor-beta superfamily of growth and differentiation factors. The encoded protein is produced in fetal Sertoli cells during male gonadal differentiation. The protein binds to the anti-Mullerian hormone receptor type 2 and causes the regression of Mullerian ducts in developing male embryos. In humans, some mutations in this gene are associated with persistent Mullerian duct syndrome (PMDS). [provided by RefSeq, Apr 2013]

Uniprot Description

Function: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.