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Western Blot (WB) (Western blotAll lanes: TRIT1 antibody at 3ug/mlLane 1: Jurkat whole cell lysateLane 2: Raji whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 53,38,50,44,19 kDaObserved band size: 53 kDa)

Rabbit anti-Human TRIT1 Polyclonal Antibody | anti-TRIT1 antibody

TRIT1 Antibody

Gene Names
TRIT1; IPT; GRO1; IPPT; MOD5; hGRO1; IPTase
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
TRIT1; Polyclonal Antibody; TRIT1 Antibody; tRNA dimethylallyltransferase; mitochondrial (EC:2.5.1.75); IPT; MOD5; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; hGRO1; tRNA isopentenyltransferase; IPTase; anti-TRIT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
441
Applicable Applications for anti-TRIT1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human tRNA dimethylallyltransferase, mitochondrial protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-54536 / sc-54536
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: TRIT1 antibody at 3ug/mlLane 1: Jurkat whole cell lysateLane 2: Raji whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 53,38,50,44,19 kDaObserved band size: 53 kDa)

Western Blot (WB) (Western blotAll lanes: TRIT1 antibody at 3ug/mlLane 1: Jurkat whole cell lysateLane 2: Raji whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 53,38,50,44,19 kDaObserved band size: 53 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7003017 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7003017 at dilution of 1:100)
Related Product Information for anti-TRIT1 antibody
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,758 Da
NCBI Official Full Name
tRNA dimethylallyltransferase, mitochondrial isoform 2
NCBI Official Synonym Full Names
tRNA isopentenyltransferase 1
NCBI Official Symbol
TRIT1
NCBI Official Synonym Symbols
IPT; GRO1; IPPT; MOD5; hGRO1; IPTase
NCBI Protein Information
tRNA dimethylallyltransferase, mitochondrial
UniProt Protein Name
tRNA dimethylallyltransferase, mitochondrial
UniProt Gene Name
TRIT1
UniProt Synonym Gene Names
IPT; MOD5; IPP transferase; IPPT; IPTase
UniProt Entry Name
MOD5_HUMAN

NCBI Description

This gene encodes a protein that that is targeted to the mitochondrion and modifies transfer RNAs (tRNAs) by adding a dimethylallyl group onto the adenine at position 37. This modification is important for maintaining the correct reading frame during protein translation. This gene is considered a tumor suppressor and its expression can decrease cell growth. Alternative splicing results in multiple transcripts variants, most of which are likely non-functional. [provided by RefSeq, Aug 2015]

Uniprot Description

TRIT1: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). Belongs to the IPP transferase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.5.1.75

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: ATP binding; metal ion binding

Biological Process: gene expression; tRNA modification; tRNA processing

Research Articles on TRIT1

Similar Products

Product Notes

The TRIT1 trit1 (Catalog #AAA7003017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIT1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the TRIT1 trit1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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