Dkk-1 active protein

Human Dkk-1

Cat. Num. AAA691712

• Gene Names: DKK1; SK; DKK-1
• Reactivity: Human
• Purity: > 97% by SDS-PAGE & HPLC analyses
• Synonyms: Dkk-1; Human Dkk-1; Dickkopf-related protein-1; Dickkopf-1; SK; Dkk-1 active protein

• Host: HEK 293 Cells
• Reactivity: Human
• Purity/Purification: > 97% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTI DNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC M RHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGY SRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PV LKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSR LH TCQRH
• Sequence Length: 266

• Biological Activity: Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200ng/ml.

Related Product Information for Dkk-1 active protein

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/betacatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/betacatenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Recombinant human DKK-1 expressed in human 293 cells is a 35-40 kDa glycoprotein containing 235 amino-acid residues.

Product Categories/Family for Dkk-1 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7110719
• NCBI GeneID: 22943
• NCBI Accession #: NP_036374.1
• NCBI GenBank Nucleotide #: NM_012242.2
• UniProt Accession #: O94907; B2RC19
• Molecular Weight: 28,672 Da
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 1
• NCBI Official Symbol: DKK1
• NCBI Official Synonym Symbols: SK; DKK-1
• NCBI Protein Information: dickkopf-related protein 1; hDkk-1; dickkopf-1 like; dickkopf 1 homolog; dickkopf-like protein 1; dickkopf related protein-1
• UniProt Protein Name: Dickkopf-related protein 1
• UniProt Gene Name: DKK1
• UniProt Synonym Gene Names: Dickkopf-1; Dkk-1; hDkk-1
• UniProt Entry Name: DKK1_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Ref.15

Subunit structure: Interacts with LRP6. Interacts (via the C-termianl Cys-rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt-mediated signaling. Ref.10 Ref.12 Ref.13

Subcellular location: Secreted.

Tissue specificity: Placenta.

Domain: The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6

By similarity.

Sequence similarities: Belongs to the dickkopf family.