Nesfatin Active Protein | NUCB2 active protein

Human Nesfatin

Cat. Num. AAA691633

• Gene Names: NUCB2; NEFA; HEL-S-109
• Reactivity: Human
• Purity: >98% (SDS-PAGE, HPLC)
• Synonyms: Nesfatin; Human Nesfatin; NUCB2 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: >98% (SDS-PAGE, HPLC)
• Form/Format: Lyophilized
• Sequence: VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKH FREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL
• Sequence Length: 420

• Biological Activity: Determined by an in vivo assay using healthy wild type male mice (C57BL/6J). Mice were treated via intraperitoneal injection once at a dose of 4 ug Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.

Related Product Information for NUCB2 active protein

Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2). Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic isletsbeta-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.

Product Categories/Family for NUCB2 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4826870
• NCBI GeneID: 4925
• NCBI Accession #: NP_005004.1
• NCBI GenBank Nucleotide #: NM_005013.2
• UniProt Accession #: P80303; Q8NFT5; A8K642; D3DQX5
• Molecular Weight: 50,196 Da
• NCBI Official Full Name: nucleobindin-2
• NCBI Official Synonym Full Names: nucleobindin 2
• NCBI Official Symbol: NUCB2
• NCBI Official Synonym Symbols: NEFA; HEL-S-109
• NCBI Protein Information: nucleobindin-2; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4; epididymis secretory protein Li 109
• UniProt Protein Name: Nucleobindin-2
• Protein Family: Nucleobindin
• UniProt Gene Name: NUCB2
• UniProt Synonym Gene Names: NEFA
• UniProt Entry Name: NUCB2_HUMAN

NCBI Description

This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

Uniprot Description

Function: Calcium-binding protein. May have a role in calcium homeostasis.Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance

By similarity.

Subcellular location: Golgi apparatus. Membrane; Peripheral membrane protein. Cytoplasm. Secreted Ref.8. Endoplasmic reticulum

By similarity. Nucleus envelope

By similarity. Note: Golgi retention is mediated by its N-terminal region. Ref.8Nesfatin-1: Secreted Ref.8.

Tissue specificity: Predominantly expressed in spleen, testis and normal stomach. Ref.3

Polymorphism: Deletion of Gln-402 is frequent.

Miscellaneous: NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.

Sequence similarities: Belongs to the nucleobindin family.Contains 2 EF-hand domains.