Typical Testing Data/Standard Curve (for reference only)
Human Nesfatin-1 ELISA Kit | NUCB2 elisa kit
Human Nesfatin-1 ELISA Kit
Gene Names
NUCB2; NEFA; HEL-S-109
Reactivity
Human
Applications
ELISA
Synonyms
Nesfatin-1; Human Nesfatin-1 ELISA Kit; NEFA; Nucleobindin-2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin; NUCB2 elisa kit
Reactivity
Human
Specificity
The Human Nesfatin-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Nesfatin-1 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Sequence Length
420
Applicable Applications for NUCB2 elisa kit
Sandwich ELISA (SE)
Samples
Cell culture supernatants, serum
Sensitivity
< 10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NUCB2 elisa kit
Principle of the Assay: The MyBioSource' Human Nesfatin-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Nesfatin-1 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Nesfatin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Nesfatin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Nesfatin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Nesfatin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.
Background/Introduction: Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,201 Da
NCBI Official Full Name
nucleobindin-2
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
NUCB2
NCBI Official Synonym Symbols
NEFA; HEL-S-109
NCBI Protein Information
nucleobindin-2; nesfatin 1; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4; epididymis secretory protein Li 109
UniProt Protein Name
Nucleobindin-2
Protein Family
UniProt Gene Name
NUCB2
UniProt Synonym Gene Names
NEFA
UniProt Entry Name
NUCB2_HUMAN
NCBI Description
This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]
Uniprot Description
NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Vesicle; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; nuclear envelope; cytosol
Molecular Function: protein binding; DNA binding; calcium ion binding
Research Articles on NUCB2
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Product Notes
The Human NUCB2 nucb2 (Catalog #AAA825097) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA825097 ELISA Kit recognizes Human NUCB2. AAA Biotech's Nesfatin-1 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the NUCB2 nucb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nesfatin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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