KLF4-Tat active protein

Human KLF4-TAT

Cat. Num. AAA691481

• Gene Names: KLF4; EZF; GKLF
• Reactivity: Human
• Purity: > 90% by SDS-PAGE & HPLC analyses
• Synonyms: KLF4-Tat; Human KLF4-TAT; Kruppel-like factor 4; EZF; GKLF; KLF4-Tat active protein

• Host: HEK 293 Cells
• Reactivity: Human
• Purity/Purification: > 90% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: MAVSDALLPS FSTFASGPAG REKTLRQAGA PNNRWREELS HMKRLP PVLP GRPYDLAAAT VATDLESGGA GAACGGSNLA PLPRRETEEF N DLLDLDFIL SNSLTHPPES VAATVSSSAS ASSSSSPSSS GPASAPS TCS FTYPIRAGND PGVAPGGTGG GLLYGRESAP PPTAPFNLAD IN DVSPSGGF VAELLRPELD PVYIPPQQPQ PPGGGLMGKF VLKASLSA PG SEYGSPSVIS VSKGSPDGSH PVVVAPYNGG PPRTCPKIKQ EAV SSCTHLG AGPPLSNGHR PAAHDFPLGR QLPSRTTPTL GLEEVLSSR D CHPALPLPPG FHPHPGPNYP SFLPDQMQPQ VPPLHYQELM PPGS CMPEEP KPKRGRRSWP RKRTATHTCD YAGCGKTYTK SSHLKAHLRT HTGEKPYHCD WDGCGWKFAR SDELTRHYRK HTGHRPFQCQ KCDRA FSRSD HLALHMKRHF GGYGRKKRRQ RRR
• Sequence Length: 479

• Biological Activity: Testing in progress.

Related Product Information for KLF4-Tat active protein

KLF4 is a member of the Kruppel-like factor (KLF) family of zinc finger transcription factors. Members of this family have in common 3 contiguous C2H2-type zinc fingers at the carboxyl terminus that comprise the DNA-binding domain. KLF4 is highly expressed in skin and gut epithelial tissues, but is also found in various other cells and tissues, including vascular endothelial cells, lymphocytes, lung, and testis. It is an important regulator of the cell cycle, transcription, and cell differentiation. Together with Sox2, Oct4, and cMyc, KLF4 can induce the reprogramming of primary human fibroblasts to a pluripotent state. KLF4 and other transcription factors can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human KLF4-TAT is a 51.7 kDa protein containing 483 amino acid residues, including 13- residue C-terminal TAT peptide.

Product Categories/Family for KLF4-Tat active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 194248077
• NCBI GeneID: 9314
• NCBI Accession #: NP_004226.3
• NCBI GenBank Nucleotide #: NM_004235.4
• UniProt Accession #: O43474; P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3; B2R8S4; B3KT79; L0R3I6; L0R4N5
• Molecular Weight: 54,671 Da
• NCBI Official Full Name: Krueppel-like factor 4
• NCBI Official Synonym Full Names: Kruppel-like factor 4 (gut)
• NCBI Official Symbol: KLF4
• NCBI Official Synonym Symbols: EZF; GKLF
• NCBI Protein Information: Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein
• UniProt Protein Name: Krueppel-like factor 4
• UniProt Gene Name: KLF4
• UniProt Synonym Gene Names: EZF; GKLF
• UniProt Entry Name: KLF4_HUMAN

Uniprot Description

Function: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Ref.10 Ref.15

Subunit structure: Interacts with POU5F1/OCT4 and SOX2

By similarity. Interacts with MUC1 (via the C-terminal domain). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a Ref.10 Ref.17

Subcellular location: Nucleus.

Domain: the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.12

Biotechnological use: POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Ref.11

Sequence similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.Contains 3 C2H2-type zinc fingers.

Sequence caution: The sequence AAB48399.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAC03462.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAD42165.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH29923.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH30811.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABG25917.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAG36271.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence EAW59020.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAW59021.1 differs from that shown. Reason: Erroneous gene model prediction.