Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) Secondary Antibody
Goat F(ab')2 Anti-Rabbit IgG (H+L), Mouse/Human ads-AP
Reactivity
Rabbit
Applications
ELISA, Immunoblot, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Gel filtration of pepsin-digested antibody.
Synonyms
Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific); Polyclonal Secondary Antibody; Goat F(ab')2 Anti-Rabbit IgG (H+L); Mouse/Human ads-AP; Goat F(ab')2 Anti-Rabbit IgG(H+L); Mouse/Human ads- Alkaline Phosphatase-Labeled; Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) (Absorbed against human & mouse immunoglobulins); Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) secondary antibody
Host
Goat
Reactivity
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human and mouse immunoglobulins.
Purity/Purification
Gel filtration of pepsin-digested antibody.
Form/Format
Alkaline Phosphatase (AP) Conjugate
Applicable Applications for Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) secondary antibody
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry
Application Notes
ELISA Suggested Dilution: 1:2,000-1:4,000
Source
Pepsin digest of goat anti-rabbit IgG (H+L).
Cross Absorption
Pooled human sera, purified human paraproteins, pooled mouse sera, and pooled mouse plasmacytoma/hybridoma proteins.
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Product Categories/Family for Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) secondary antibody
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Product Notes
The Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) (Catalog #AAA674765) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat F(ab')2 Anti-Rabbit IgG (H+L), Mouse/Human ads-AP reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry. ELISA Suggested Dilution: 1:2,000-1:4,000. Researchers should empirically determine the suitability of the Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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