Testing Data
(FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-human IgG4 Fc-PE (MBS674080))
Mouse Anti-Human IgG4 (gamma 4 chain specific) Secondary Antibody
Mouse Anti-Human IgG4 (Fc)-BIOT
Reactivity
Human
Synonyms
Mouse Anti-Human IgG4 (gamma 4 chain specific); Monoclonal Secondary Antibody; Mouse Anti-Human IgG4 (Fc)-BIOT; Mouse Anti-Human IgG4 (Fc) - Biotin; Mouse Anti-Human IgG4 (gamma 4 chain specific) secondary antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG1 kappa
Clone Number
HP6025
Specificity
Human IgG4 Fc; Mr 146 kDa
Form/Format
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3.
Application Notes
ELISA – Quality tested 2-9
FLISA – Quality tested
Flow Cytometry – Reported in literature 15,18,19
Immunohistochemistry-Frozen Sections – Reported in literature 10
Immunohistochemistry-Paraffin Sections – Reported in literature 5,7,10,11
Immunocytochemistry – Reported in literature 12
Western Blot – Reported in literature 3,13-16
Microarray – Reported in literature 17
Multiplex – Reported in literature 2,20-22
Purification – Reported in literature 15
ELISA
AP conjugate 1: 1,000-1: 2,000
HPR conjugate 1: 4,000-1: 8,000
BIOT conjugate 1:5,000-1: 10,000
ELISA
FITC , AF488 and AF555 conjugates 1:200-1:400
PE and AF647 conjugates <= 1 mg/ml
Other applications
Since applications vary, you should determine the optimum working dilution for the production that is appropriate for your specific need.
FLISA – Quality tested
Flow Cytometry – Reported in literature 15,18,19
Immunohistochemistry-Frozen Sections – Reported in literature 10
Immunohistochemistry-Paraffin Sections – Reported in literature 5,7,10,11
Immunocytochemistry – Reported in literature 12
Western Blot – Reported in literature 3,13-16
Microarray – Reported in literature 17
Multiplex – Reported in literature 2,20-22
Purification – Reported in literature 15
ELISA
AP conjugate 1: 1,000-1: 2,000
HPR conjugate 1: 4,000-1: 8,000
BIOT conjugate 1:5,000-1: 10,000
ELISA
FITC , AF488 and AF555 conjugates 1:200-1:400
PE and AF647 conjugates <= 1 mg/ml
Other applications
Since applications vary, you should determine the optimum working dilution for the production that is appropriate for your specific need.
Immunogen
Human IgG4 myeloma protein
Warning
Some reagents contain sodium azide.
Preparation and Storage
Store at 2-8°C
Testing Data
(FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-human IgG4 Fc-PE (MBS674080))
Testing Data
(FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-human IgG4 Fc-PE (MBS674080))
Product Categories/Family for Mouse Anti-Human IgG4 (gamma 4 chain specific) secondary antibody
Similar Products
Product Notes
The Mouse Anti-Human IgG4 (gamma 4 chain specific) (Catalog #AAA674079) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IgG4 (Fc)-BIOT reacts with Human and may cross-react with other species as described in the data sheet. ELISA – Quality tested 2-9 FLISA – Quality tested Flow Cytometry – Reported in literature 15,18,19 Immunohistochemistry-Frozen Sections – Reported in literature 10 Immunohistochemistry-Paraffin Sections – Reported in literature 5,7,10,11 Immunocytochemistry – Reported in literature 12 Western Blot – Reported in literature 3,13-16 Microarray – Reported in literature 17 Multiplex – Reported in literature 2,20-22 Purification – Reported in literature 15 ELISA AP conjugate 1: 1,000-1: 2,000 HPR conjugate 1: 4,000-1: 8,000 BIOT conjugate 1:5,000-1: 10,000 ELISA FITC, AF488 and AF555 conjugates 1:200-1:400 PE and AF647 conjugates <= 1 mg/ml Other applications Since applications vary, you should determine the optimum working dilution for the production that is appropriate for your specific need. . Researchers should empirically determine the suitability of the Mouse Anti-Human IgG4 (gamma 4 chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse Anti-Human IgG4 (gamma 4 chain specific), Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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