Testing Data (TD)
Goat Anti-Mouse IgG1 (gamma 1 chain specific) Secondary Antibody
Goat Anti-Mouse IgG1, Human ads-APC
Reactivity
Mouse
Purity
Affinity chromatography on mouse IgG1 covalently linked to agarose
Synonyms
Goat Anti-Mouse IgG1 (gamma 1 chain specific); Polyclonal Secondary Antibody; Goat Anti-Mouse IgG1; Human ads-APC; Human ads - Allophycocyanin; Goat Anti-Mouse IgG1 (gamma 1 chain specific) secondary antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the heavy chain of mouse IgG1 .
Purity/Purification
Affinity chromatography on mouse IgG1 covalently linked to agarose
Form/Format
The R-phycoerythrin (R-PE), allophycocyanin (APC) and conjugates are supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent.
Application Notes
Quality tested applications include
ELISA 1-8
FLISA
FC 6,9-15
Other referenced applications include –
ELISPOT 4,6,7
IHC-FS 3,9,16,17
IHC-PS 1,16,18,19
IHC-WM 25
ICC 14,20,21
EM 17
WB 2,5,13,22,23
Multiplex 26
SPR 24
Working dilutions:
ELISA
AP conjugate 1:2,000 – 1:4,000
HRP conjugate 1:4,000 – 1:8,000
BIOT conjugate 1:5,000 – 1:20,000
FLISA
FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 – 1:400
PE, APC, CY5, and AF647 conjugates <= 1 ug/mL
Flow Cytometry
FITC, BIOT, and AF488 conjugates <= 1 ug/106 cells
PE, APC, CY5, APC/CY7, and AF647 conjugates <= 0.1 ug/106 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL
Other Applications
Since applications vary, you should determine the optimum working dilution for the product that isappropriate for your specific need
ELISA 1-8
FLISA
FC 6,9-15
Other referenced applications include –
ELISPOT 4,6,7
IHC-FS 3,9,16,17
IHC-PS 1,16,18,19
IHC-WM 25
ICC 14,20,21
EM 17
WB 2,5,13,22,23
Multiplex 26
SPR 24
Working dilutions:
ELISA
AP conjugate 1:2,000 – 1:4,000
HRP conjugate 1:4,000 – 1:8,000
BIOT conjugate 1:5,000 – 1:20,000
FLISA
FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 – 1:400
PE, APC, CY5, and AF647 conjugates <= 1 ug/mL
Flow Cytometry
FITC, BIOT, and AF488 conjugates <= 1 ug/106 cells
PE, APC, CY5, APC/CY7, and AF647 conjugates <= 0.1 ug/106 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL
Other Applications
Since applications vary, you should determine the optimum working dilution for the product that isappropriate for your specific need
Host Note
Pooled antisera from goats hyperimmunized with mouse IgG1
Cross Adsorption
Mouse IgG2a, IgG2b, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species.
Preparation and Storage
Store at 2-8°C. Do not freeze!
Testing Data (TD)
Testing Data (TD)
Product Categories/Family for Goat Anti-Mouse IgG1 (gamma 1 chain specific) secondary antibody
Similar Products
Product Notes
The Goat Anti-Mouse IgG1 (gamma 1 chain specific) (Catalog #AAA674458) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Mouse IgG1, Human ads-APC reacts with Mouse and may cross-react with other species as described in the data sheet. Quality tested applications include ELISA 1-8 FLISA FC 6,9-15 Other referenced applications include – ELISPOT 4,6,7 IHC-FS 3,9,16,17 IHC-PS 1,16,18,19 IHC-WM 25 ICC 14,20,21 EM 17 WB 2,5,13,22,23 Multiplex 26 SPR 24 Working dilutions: ELISA AP conjugate 1:2,000 – 1:4,000 HRP conjugate 1:4,000 – 1:8,000 BIOT conjugate 1:5,000 – 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 – 1:400 PE, APC, CY5, and AF647 conjugates <= 1 ug/mL Flow Cytometry FITC, BIOT, and AF488 conjugates <= 1 ug/106 cells PE, APC, CY5, APC/CY7, and AF647 conjugates <= 0.1 ug/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL Other Applications Since applications vary, you should determine the optimum working dilution for the product that isappropriate for your specific need. Researchers should empirically determine the suitability of the Goat Anti-Mouse IgG1 (gamma 1 chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Mouse IgG1 (gamma 1 chain specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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