Rat anti-Mouse CD25/IL-2R alpha Monoclonal Antibody
Rat Anti-Mouse CD25/IL-2Ra-UNLB
The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.^2 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.^1, 3, 4 It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220^low TdT^- sIg^- pre-B II and CD45R/B220^low TdT^- sIgM^+sIgD^- immature B cells, but not on the CD45R/B220^low TdT^+ sIg^- pro-B/pre-B I stage nor on CD45R/B220^high TdT^- sIgM^+sIgD^+ mature B cells.^5, 6 CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.^6, 7 The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7 (Cat. No. 1600-XX). While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antobody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.^3, 8, 9
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
2. Leonard, W.J., et al. 1984. Nature 311:626.
3. Ortega, G., R. J. Robb, E. M. Shevach, and T. R. Malek. 1984. J. Immunol. 133:1970.
4. Malek, T. R., J. A. Schmidt, and E. M. Shevach. 1985. J. Immunol. 134:2405.
5. Rolink, A., U. Grawunder, T.H. Winkler, H. Karasuyama, and F. Melchers. 1994. Int. Immunol. 6:1257.
6. Chen, J., A. Ma, F. Young, and F. W. Alt. 1994. International. Immunology 6:1265.
7. Habu, S., K. Okumura, T. Diamantstein, and E. M. Shevach. 1985. Eur. J. Immunol. 15:456.
8. Moreau, J. L., M. Nabholz, T. Diamantstein, T. R. Malek, E. Shevach, and J. Theze. 1987. Eur. J. Immunol. 17:929.
9. Lorenzo, F., C. Jaulin, N. Vita., P. Froussard, P. Ferrara, D. Jankovic, and J. Theze. J. Immunol. 147:2970.