Activity
(Figure 1 : ACE2 (human)(rec.) (32-190010) binds with high affinity to the Spike (RBD) protein of the virus SARS-CoV-2. Method: ACE2 (human)(rec.) (32-190010 ) is coated on an ELISA plate at 1 ?g/ml overnight at 4°C. Spike (SARS-CoV-2):Fc (human)(RBD)(rec.) is added (starting at a concentration of 500 ng/ml with a twofold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).)
ACE2 active protein
Human ACE2 Protein (DYKDDDDK Tag)
Purity
>=95% (SDS-PAGE)
Synonyms
ACE2; Human ACE2 Protein (DYKDDDDK Tag); ACE2 active protein
Host
HEK293 Cells
Purity/Purification
>=95% (SDS-PAGE)
Form/Format
1mg/ml after reconstitution. Reconstitute with 50ul endotoxin-free water.
Application Notes
~110kDa (SDS-PAGE); ~240kDa (Dimer, by SEC). Binds to the Spike protein of the coronavirus SARS-CoV-2. Endotoxin content: <0.01EU/ug purified protein (LAL test).
Tag Information
Extracellular domain of human ACE2 (aa 18-740) are fused at the C-terminus to a DYKDDDDK-tag.
Preparation and Storage
After opening, prepare aliquots and store at -20 degree C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Stable for at least 6 months after receipt when stored at -20 degree C. Working aliquots are stable for up to 3 months when stored at -20 degree C.
Activity
(Figure 1 : ACE2 (human)(rec.) (32-190010) binds with high affinity to the Spike (RBD) protein of the virus SARS-CoV-2. Method: ACE2 (human)(rec.) (32-190010 ) is coated on an ELISA plate at 1 ?g/ml overnight at 4°C. Spike (SARS-CoV-2):Fc (human)(RBD)(rec.) is added (starting at a concentration of 500 ng/ml with a twofold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).)
Activity
(Figure 1 : ACE2 (human)(rec.) (32-190010) binds with high affinity to the Spike (RBD) protein of the virus SARS-CoV-2. Method: ACE2 (human)(rec.) (32-190010 ) is coated on an ELISA plate at 1 ?g/ml overnight at 4°C. Spike (SARS-CoV-2):Fc (human)(RBD)(rec.) is added (starting at a concentration of 500 ng/ml with a twofold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).)
Related Product Information for ACE2 active protein
Angiotensin-converting enzyme2 (ACE2) is an ectoenzyme (carboxypeptidase) with an extracellular catalytic domain that predominantly localizes at the plasma membrane and is thereby able to hydrolyze circulating peptides. ACE2 has approximately 42% sequence identity with ACE, and its cytoplasmic and transmembrane domains show 48% homology to the protein collectrin that plays a critical role in the amino acid absorption of the kidney. ACE2 converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. ACE2 is involved in the regulation of systemic blood pressure and has direct effects on cardiac functions. It is expressed predominantly in endothelial cells of the lung, gut, heart and kidney. ACE2 together with the protease TMPRSS2 acts as a functional receptor for SARS coronavirus as well as for the new highly pathogenic coronavirus, 2019-nCoV/SARS-CoV-2, which is cause for pneumonia COVID-19.
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Product Notes
The ACE2 (Catalog #AAA669403) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is Extracellular domain of human ACE2 (aa 18-740) are fused at the C-terminus to a DYKDDDDK-tag. ~110kDa (SDS-PAGE); ~240kDa (Dimer, by SEC). Binds to the Spike protein of the coronavirus SARS-CoV-2. Endotoxin content: <0.01EU/ug purified protein (LAL test). Researchers should empirically determine the suitability of the ACE2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACE2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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