Flow Cytometry (FC/FACS)
(Figure-1: Cell surface flowcytometric staining of mGITR (Clone: DTA-1) in mouse Thymocytes. Mouse Thymocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mGITR using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Rat anti-Mouse GITR Monoclonal Antibody | anti-GITR antibody
Monoclonal Antibody to GITR (Clone: DTA-1)
Gene Names
Tnfrsf18; AITR; Gitr
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
PurifiedProtein G Chromatography
Synonyms
GITR; Monoclonal Antibody; Monoclonal Antibody to GITR (Clone: DTA-1); Tumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; CD357; anti-GITR antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b, Lambda
Clone Number
DTA-1
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
228
Applicable Applications for anti-GITR antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 0.5-1 ug/10^6
Antigen
Gitr
Immunogen Information
Mouse CD25 and CD4 positive T cells were taken as the immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeat freezing and thawing
For long-term storage, store at -20 degree C.
Avoid repeat freezing and thawing
Flow Cytometry (FC/FACS)
(Figure-1: Cell surface flowcytometric staining of mGITR (Clone: DTA-1) in mouse Thymocytes. Mouse Thymocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mGITR using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Flow Cytometry (FC/FACS)
(Figure-1: Cell surface flowcytometric staining of mGITR (Clone: DTA-1) in mouse Thymocytes. Mouse Thymocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mGITR using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Flow Cytometry (FC/FACS)
(Figure-2: Cell surface flowcytometric staining of mGITR (Clone: DTA-1) in mouse Splenocytes. Mouse Splenocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mGITR using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Flow Cytometry (FC/FACS)
(Figure-2: Cell surface flowcytometric staining of mGITR (Clone: DTA-1) in mouse Splenocytes. Mouse Splenocytes were first stained with Anti-mCD3 APC conjugated, mCD3 APC positive cells were gated and further analyzed for mGITR using 0.5 ug/10^6. Goat anti-Rat PE secondary antibody was used.)
Product Categories/Family for anti-GITR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,106 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 18 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 18
NCBI Official Symbol
Tnfrsf18
NCBI Official Synonym Symbols
AITR; Gitr
NCBI Protein Information
tumor necrosis factor receptor superfamily member 18
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 18
UniProt Gene Name
Tnfrsf18
UniProt Synonym Gene Names
Gitr
Uniprot Description
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway ().
Research Articles on GITR
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Product Notes
The GITR tnfrsf18 (Catalog #AAA668984) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to GITR (Clone: DTA-1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GITR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the GITR tnfrsf18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GITR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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