Rabbit Daxx Polyclonal Antibody | anti-DAXX antibody

Polyclonal Antibody to Daxx

Cat. Num. AAA668616

• Gene Names: DAXX; DAP6; EAP1; BING2
• Reactivity: Dog, Rat, Human; Predicted: Mouse
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Serum
• Synonyms: Daxx; Polyclonal Antibody; Polyclonal Antibody to Daxx; DAXX; BING2; DAP6; EAP1; ETS1-associated protein 1; Fas death domain-associated protein; Antibody to Daxx; Anti-Daxx Antibody; Daxx Antibody; anti-DAXX antibody

• Host: Rabbit
• Reactivity: Dog, Rat, Human; Predicted: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum
• Form/Format: 50 ul sera
• Sequence Length: 740

• Applicable Applications for anti-DAXX antibody: Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)
• Application Notes: WB: 1: 1000-1: 2000; IHC (paraffin): 1: 1000-1: 5000; IP: 1: 50-1: 200
• Immunogen Information: A synthetic peptide of Daxx protein (amino acids 272-291 ERLINKPGPDTFPDYGDVLR) was used as immunogen for this antibody
• Antigen: Daxx
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.; For long-term storage, store at -20 degree C.; Avoid repeated freeze and thaw cycles.

Immunohistochemistry (IHC)

(Fig: 1 Formalin-fixed paraffin-embedded tissue sections of human prostate cancer (A and B) stained for Daxx expression using at 1: 2000. Hematoxylin-eosin counterstain.)

Related Product Information for anti-DAXX antibody

Daxx (death-domain-associated protein) was originally identified as a cytoplasmic protein that binds to the death domain of the transmembrane death receptor Fas. It is now that known that the a large porportion of Daxx molecules are nuclear and associate with the promyelocytic leukaemia nuclear body (PML-NB) and other subnuclear domains.Daxx is ubiquitously expressed, and particularly high levels of Daxx have been reported in the thymus and testes. At the cellular level Daxx may be found in cytoplasm and within heterochromatic regions of the nucleus. Daxx has been reported to interact and co-localize with Pml in the nucleus of tumor cell lines and primary cells. Daxx is thought to have both pro- and anti-apoptotic functions depending on the stimulus and the cell type. Although the mechanisms remain to be fully elucidated, research indicates that Daxx plays a role in the pathology of human diseases including cancer and neurodegerative disorders.

Product Categories/Family for anti-DAXX antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 24636785
• NCBI GeneID: 1616
• UniProt Accession #: Q9UER7; O14747; O15141; O15208; Q5STK9; Q9BWI3; B4E1I3; F5ANJ6; F5ANJ7; F5H082
• Molecular Weight: 75,563 Da
• NCBI Official Full Name: Death domain-associated protein 6
• NCBI Official Synonym Full Names: death domain associated protein
• NCBI Official Symbol: DAXX
• NCBI Official Synonym Symbols: DAP6; EAP1; BING2
• NCBI Protein Information: death domain-associated protein 6
• UniProt Protein Name: Death domain-associated protein 6
• Protein Family: Death domain-associated protein
• UniProt Gene Name: DAXX
• UniProt Synonym Gene Names: BING2; DAP6; hDaxx; EAP1

NCBI Description

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Apoptosis; Nuclear receptor co-regulator; Nucleolus; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p21.32

Cellular Component: chromosome, pericentric region; cytoplasm; cytosol; nucleoplasm; nucleus; PML body

Molecular Function: androgen receptor binding; enzyme binding; heat shock protein binding; histone binding; p53 binding; protein binding; protein kinase activator activity; protein kinase binding; protein N-terminus binding; receptor signaling protein activity; transcription corepressor activity; ubiquitin protein ligase binding

Biological Process: activation of JNK activity; androgen receptor signaling pathway; apoptosis; chromatin remodeling; induction of apoptosis via death domain receptors; negative regulation of transcription, DNA-dependent; nucleosome assembly; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase activity; regulation of protein ubiquitination; regulation of transcription, DNA-dependent