Mouse anti-Human PDL-1 Monoclonal Antibody | anti-CD274 antibody

PDL-1

Cat. Num. AAA668222

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein G purification
• Synonyms: PDL-1; Monoclonal Antibody; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1; anti-CD274 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, Kappa
• Clone Number: ABM5F25
• Purity/Purification: Protein G purification
• Form/Format: Purified
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 176

• Applicable Applications for anti-CD274 antibody: Flow cytometry (FC/FACS)
• Application Notes: FACS analysis: 0.5-1 ug/10^6 cells; Western blot analysis: 2-4 ug/ml; Immunohistochemical analysis: 5-10 ug/ml
• Immunogen: A partial length recombinant protein of PDL-1 (amino acid 13-224) was used as the immunogen for this antibody.
• Buffer: PBS containing 0.05% BSA and 0.05% sodium azide.
• Target Modification: No
• Produced from Ascites: No
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles.

Flow Cytometry

(Cell Surface flow analysis of PD-L 1 in 3 day-PHA treated human PBMC cells using 1 ug/10^6 cells of PD-L 1 antibody (Clone: ABM5F25). Green represents isotype control; red represents anti-PD-L 1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

Flow Cytometry

(Cell surface flow analysis of PD-L 1 in CHO-PD-L 1 transfected cell line using 0.5ug/10^6 cells of PD-L 1 antibody (Clone: ABM5F25). Green represents isotype control;red represents anti-PD-L 1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

Flow Cytometry

(Western blot analysis of PDL 1. Anti-PD-L 1 antibody (Clone: ABM5F25) was tested at 0.5 ug/ml on Recombinant lysate.)

Western blot

(Western blot analysis of POL 1. Anti-PO-L 1 antibody (Clone: ABM5F25) was tested at 2 ug/ml on (1) Daudi and (2) HepG2 Iysates.)

Immunohistochemical

(Immunohistochemical analysis of PO-L 1 in Hodkin's Lymphoma tissue using POL 1 antibody (Clone: ABM5F25) at 5 ug /ml.)

Related Product Information for anti-CD274 antibody

PD-L 1 (CD274/B7-H1 ) is a critical membrane-bound costimulatory molecule belongs to the B7 superfamily that inhibits immune responses through its receptor, PD-1 and PD-L 1 playa key role in the pathogenesis of inflammatory diseases (programmed death 1). It is widely expressed in the mononuclear phagocy1e system (MPS), may co-stimulate T cells and regulates inflammatory responses. PD-L 1 exerts inflammation regulatory functions via a negative co-stimulatory effect on T cell functions to inhibit cytokine secretion , facilitate apoptosis of activated T cells and induce T cell anergy. Aberrant expression and dysregulation of CD274 have been reported during bacterial infection, inflammation and in numerous autoimmune diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 390979639
• NCBI GeneID: 29126
• NCBI Accession #: NP_001254635.1
• NCBI GenBank Nucleotide #: NM_001267706.1
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform b
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• Protein Family: Programmed cell death
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_HUMAN

NCBI Description

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: endomembrane system; external side of plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction; immune response; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; positive regulation of cell migration; positive regulation of T cell proliferation; regulation of activated T cell proliferation; response to cytokine stimulus; signal transduction; T cell costimulation