Mouse anti-Human PD-L1 Monoclonal Antibody | anti-PD-L1 antibody

Anti-PD-L1 Monoclonal Antibody (Clone: ABM5F25)

Cat. Num. AAA847391

• Gene Names: CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Protein G Chromatography
• Synonyms: PD-L1; Monoclonal Antibody; Anti-PD-L1 Monoclonal Antibody (Clone: ABM5F25); CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1; anti-PD-L1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone Number: ABM5F25
• Specificity: Monoclonal antibody for the detection of PD-L1 in human cells and tissues
• Purity/Purification: Protein G Chromatography
• Form/Format: Colorless Liquid. 0.5mg/ml in Supplied in PBS containing 0.05% BSA and 0.05% Sodium azide.

• Applicable Applications for anti-PD-L1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: WB: 2-4 mg/ml; FC/FACS: 0.5-1mg/106 cells; IHC: 5-10mg/ml
• Immunogen: A partial length recombinant protein of PD-L1 (amino acid 13-224) was used as the immunogen
• Preparation and Storage: Store at 4 degree C; The antibody solution should be gently mixed before use; Shelf life: ~6 months

Flow Cytometry (FC/FACS)

Immunohistochemistry (IHC)

Western Blot (WB)

Related Product Information for anti-PD-L1 antibody

PD-L1 (CD274/B7-H1) is a critical membrane-bound costimulatory molecule belongs to the B7 superfamily that inhibits immune responses through its receptor, PD-1 and PD-L1 play a key role in the pathogenesis of inflammatory diseases (programmed death 1). It is widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells and regulates inflammatory responses. PD-L1 exerts inflammation regulatory functions via a negative co-stimulatory effect on T cell functions to inhibit cytokine secretion, facilitate apoptosis of activated T cells and induce T cell anergy. Aberrant expression and dysregulation of CD274 have been reported during bacterial infection, inflammation and in numerous autoimmune diseases.

Product Categories/Family for anti-PD-L1 antibody

Immune Checkpoint regulator; Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 390979639
• NCBI GeneID: 29126
• NCBI Accession #: NP_001254635.1
• NCBI GenBank Nucleotide #: NM_001267706.1
• UniProt Accession #: Q9NZQ7; Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
• Molecular Weight: 33,275 Da
• NCBI Official Full Name: programmed cell death 1 ligand 1 isoform b
• NCBI Official Synonym Full Names: CD274 molecule
• NCBI Official Symbol: CD274
• NCBI Official Synonym Symbols: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
• NCBI Protein Information: programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: CD274
• UniProt Synonym Gene Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_HUMAN

Uniprot Description

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: plasma membrane; integral to membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction