Mouse anti-Human CD62E Monoclonal Antibody | anti-CD62E antibody

anti-human CD62E-Biotin

Cat. Num. AAA666588

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD62E; Monoclonal Antibody; anti-human CD62E-Biotin; Monoclonal anti-human CD62E (E-selectin)/Biotin; anti-CD62E antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: HAE-1f
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 0.5 mg/ml (varies by lot)

• Applicable Applications for anti-CD62E antibody: Flow Cytometry (FC/FACS)
• Performance: Cultured human umbilical cord vein endothelial cells (HUVEC) were stimulated 4 hours with 10 ng/ml Phorbol 12-Myristate 13-Acetate. Five x 105 cells per tube were harvested, washed and incubated 45 minutes on ice with 80 ul of anti-CD62E/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. A net 62% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD62E antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: TNF activated HUVEC
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD62E antibody

Information: Human CD62E (E-selectin) is an adhesion molecule expressed on activated endothelium and facilitates recruitment of leukocytes to sights of acute inflammation and immunological events. CD62E has affinity to LewisX (1). Antibody HAE-1f recognizes the lectin domain of CD62E (2). Antibody HAE-1f blocks the function of CD62E.

References

(1). T.P. Kogan, et al, (1995) J Biol Chem 270: 14047-14055. (2). L. Tu, et al, (1996) J Immunol 157: 3995-4004. (3). Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 416-419.