Testing Data
Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody
anti-human CD3-Purified Preservative Free
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified Preservative Free
Synonyms
CD3; Monoclonal Antibody; anti-human CD3-Purified Preservative Free; Monoclonal anti-human CD3e; anti-CD3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
UCHT1
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
167
Applicable Applications for anti-CD3 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), PS
Performance
Five x 105 ficoll prepared human PBMC were washed and incubated 45 minutes on ice with 80 ul of anti-CD3 antibody at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS using a lymphocyte gate. An 83% sub population of cells stained positive for CD3 with a mean shift of 1.99 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human thymocytes/Sezary T cells
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD3 antibody
Information: The human CD3/T cell receptor (TcR) complex is made up of at least five CD3 proteins (gamma, delta, epsilon, h, z) in association with either alpha/beta or gamma/delta proteins of the TcR. The TcR recognizes antigens in association with MHC molecules after which protein chains of the CD3 complex mediate activation signals triggered by TcR antigen binding. CD3 is expressed on greater than 95% of circulating human peripheral T cells. Antibody UCHT1 recognizes the 20 kd episilon chain of the CD3 molecule complex. Antibody UCHT1 will activate T cells expressing CD3epsilon.
References
P.C. L. Beverly & R.E. Callard, Eur J Immunol (1981) 11: 329-334. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 290-314. A. Salmeron, et al, (1991) J Immunol 147: 3047-3052. G. Thibault & P. Bardos, (1995) J Immunol 154: 3814-3820.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
18,897 Da
NCBI Official Full Name
cd3
UniProt Protein Name
CD3gamma/delta
Protein Family
UniProt Gene Name
cd3g
UniProt Entry Name
P79951_XENLA
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Product Notes
The CD3 cd3g (Catalog #AAA666464) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD3-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), PS. Researchers should empirically determine the suitability of the CD3 cd3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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