Mouse anti-Human DR3 Monoclonal Antibody | anti-DR3 antibody

anti-human DR3-Biotin

Cat. Num. AAA666208

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• Reactivity: Human
• Applications: ELISA
• Synonyms: DR3; Monoclonal Antibody; anti-human DR3-Biotin; Monoclonal anti-human DR3/Biotin; anti-DR3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: ANC2D12
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-DR3 antibody: ELISA (EIA)
• Performance: Anti-DR3/Biotin was tested for its ability to detect captured DR3-muIg in EIA. Goat anti-Mouse Ig coated plate was washed and incubated with varying amounts of DR3-muIg. Plate wells were flooded with 50ul of a mixture of 200ug/ml Mouse IgG (Sigma tech grade) and 200ug/ml monoclonal Mouse IgG1. This was to block remaining active GAM sites. Fifty ul of anti-DR3/Biotin was added and incubated at a final concentration of 1ug/ml. Plates were washed twice and incubated with Streptavidin/HRP after which they were washed three times, developed with TMB/H202 substrate/chromagen, fixed with 2N H2SO4 and read at 450nm. Captured DR3-muIg was detectable at 5 ng/ml.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Recombinant DR3
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-DR3 antibody

Information: Human DR3 (TRAMP, LARD) was designated TNF receptor superfamily member 25. TL1A is a ligand which can induce apoptosis through a cytoplasmic death domain similar to the CD178-CD95 (FasL-Fas) interaction. Alternatively, engagement of DR3 on T cells can synergize with other activating signals to enhance IFN? production. Clone ANC2D12 binds specifically to recombinant DR3 in EIA, but does not inhibit DR3 binding to TL1A.

References

(1) A Kaptein, et al. (2000) FEBS Lett 485:135. (2) T Migone, et al. (2002) Immunity 16:479. (3) S Targin, et al. (2004) 172: 7002.

NCBI and Uniprot Product Information

• NCBI GI #: 37181738
• NCBI GeneID: 8718
• Molecular Weight: 29,111 Da
• NCBI Official Full Name: DR3
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25; protein WSL-1; death receptor beta; apoptosis inducing receptor; apoptosis-inducing receptor AIR; apoptosis-mediating receptor DR3; apoptosis-mediating receptor TRAMP; death domain receptor 3 soluble form; lymphocyte-associated receptor of death; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD
• UniProt Entry Name: TNR25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytosol

Molecular Function: tumor necrosis factor receptor activity; receptor activity

Biological Process: regulation of apoptosis; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction