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Testing Data (Example: The Multiple Tag Cell Lysate was loaded on a SDS gel and the 40kD fusion protein was detected using a His-Tag Western Kit.)

Multiple Tag Cell Lysate

Multiple Tag Cell Lysate

Applications
Western Blot, Gel Super Shift Assay
Purity
Lysate
Synonyms
Multiple Tag Cell Lysate; Multiple Tag Cell Lysate lysate
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Lysate
Form/Format
Supplied as a lyophilized powder. Reconstitute with 200ul sterile dH2O.
Applicable Applications for Multiple Tag Cell Lysate lysate
Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Western Blot and Dot Blot. applications not tested.
Dilution: Western Blot: 0.02-0.5ul per lane of minigel. Dilute with 1X SDS Loading Buffer.
Preparation and Storage
Reconstitute to nominal volume by adding sterile dH2O. Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. Reconstituted product is stable for 12 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Example: The Multiple Tag Cell Lysate was loaded on a SDS gel and the 40kD fusion protein was detected using a His-Tag Western Kit.)

Testing Data (Example: The Multiple Tag Cell Lysate was loaded on a SDS gel and the 40kD fusion protein was detected using a His-Tag Western Kit.)
Related Product Information for Multiple Tag Cell Lysate lysate
Multiple Tag Cell Lysate is whole E. coli cell lysate comprised of about 30% specially designed fusion protein, which act as a positive control for Western blot or Dot blot. The fusion protein is a 52kD protein containing 16 commonly used tags, which are, in order, Trx, T7, HSV, C-Myc, SVS-g, Glu-Glu, V5, E tag, DYKDDDDK, S-tag, HA, KT3, E2, AU1, AU5, and His tag.
Product Categories/Family for Multiple Tag Cell Lysate lysate

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Product Notes

The Multiple Tag Cell Lysate (Catalog #AAA657125) is a Lysate produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Multiple Tag Cell Lysate can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in Western Blot and Dot Blot. applications not tested. Dilution: Western Blot: 0.02-0.5ul per lane of minigel. Dilute with 1X SDS Loading Buffer. Researchers should empirically determine the suitability of the Multiple Tag Cell Lysate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Multiple Tag Cell Lysate, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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