Griffonia simplicifolia Lectin I Isolectin B4 Lectin
Griffonia simplicifolia Lectin I Isolectin B4 (Bandeiraea simplicifolia) (GSL I-isolectin B4, GS I-B4)
Purity
Homogeneous by SDS-PAGE
Synonyms
Griffonia simplicifolia Lectin I Isolectin B4; Griffonia simplicifolia Lectin I Isolectin B4 (Bandeiraea simplicifolia) (GSL I-isolectin B4; GS I-B4); Griffonia simplicifolia Lectin I Isolectin B4 lectin
Purity/Purification
Homogeneous by SDS-PAGE
Form/Format
Supplied as a lyophilized powder. Reconstitute in 10mM HEPES, pH 8.5, 0.1mM Ca++. For preserving solutions, stored at 4ºC, 0.08% sodium azide can be used
Application Notes
MBS656234 will agglutinate blood group B glutaraldehyde-fixed human red blood cells at a concentration of 2ug/ml. No agglutination of blood group A, glutaraldehyde-fixed, human red blood cells occurred at concentrations up to 0.1mg/ml.
Inhibiting Sugar
500mM galactose or 100mM raffinose
Preparation and Storage
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Griffonia simplicifolia Lectin I Isolectin B4 lectin
GSL I is a family of glycoproteins with molecular weights of ~114kD. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.
GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.
Product Categories/Family for Griffonia simplicifolia Lectin I Isolectin B4 lectin
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Product Notes
The Griffonia simplicifolia Lectin I Isolectin B4 (Catalog #AAA656234) is a Lectin and is intended for research purposes only. The product is available for immediate purchase. MBS656234 will agglutinate blood group B glutaraldehyde-fixed human red blood cells at a concentration of 2ug/ml. No agglutination of blood group A, glutaraldehyde-fixed, human red blood cells occurred at concentrations up to 0.1mg/ml. Researchers should empirically determine the suitability of the Griffonia simplicifolia Lectin I Isolectin B4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Griffonia simplicifolia Lectin I Isolectin B4, Lectin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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