CpG-B DNA, K-type, BioGenomics DNA Ladder
CpG-B DNA, Human, Mouse, K-type, BioGenomics
Applications
ELISA
Purity
PurifiedPurified
Synonyms
CpG-B DNA; K-type; BioGenomics; Human; Mouse; BioGenomics dna ladder
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a lyophilized powder. Endotoxin levels are undetectable. Reconstitute with sterile ddH2O.
Applicable Applications for CpG-B DNA, K-type, BioGenomics dna ladder
ELISA (EL/EIA)
Application Notes
Suitable for use in biological assays in vitro to activate cells and as an immune modulating agent.
Dilution: In vitro Stimulation: 0.05-3uM
Dilution: In vitro Stimulation: 0.05-3uM
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for CpG-B DNA, K-type, BioGenomics dna ladder
ODN 2006 is a prototype of the class of CpG-B oligodeoxynucleotides (ODN), also known as 'K'-type ODN, with a full phosphorothioate (PS) backbone. It is particularly effective for activating B cells.The vertebrate immune system has evolved innate immune defense pattern recognition receptors (PRRs) that detect unmethylated cytosine-phosphate-guanine (CpG) motifs within bacterial DNA. Cellular activation by CpG motifs occurs via the Toll signal pathway. The Toll-like receptor-9 (TLR9, CD289) appears to be a major component of the CpG-DNA receptor, acting by direct binding to CpG-DNA, which triggers the induction of cell signaling pathways including the mitogen activated protein kinase (MAPKs) and NFkB, leading to stimulation of various cells of the immune system. The human TLR9 is expressed in B cells and plasmacytoid dendritic cells (PDC). Mice also express TLR9 in the myeloid compartment. Optimal sequences for activating TLR9 vary among species. Synthetic ODN contain CpG-DNA motifs mimicking the immunostimulatory effects of bacterial DNA and can, therefore, be used as immunoprotective agents, vaccine adjuvants and anti-allergic agents. CpG ODN also affects immune tolerance and autoimmunity. Different classes of CpG ODN are characterized each with distinct effects on the immune response: CpG-A ('D'-type), CpG-B ('K'-type), and CpG-C. CpG-B ODN are characterized by a full phosphorothioate backbone with one or more CpG motifs without poly -G motifs. CpG-B ODN are weak inducers of IFN-alpha but are very potent Th1 adjuvants and strong B cell response stimulators. CpG-B ODN promote survival, activation, and maturation of both monocyte derived dendritic cells and PDC.
Product Categories/Family for CpG-B DNA, K-type, BioGenomics dna ladder
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Product Notes
The CpG-B DNA, K-type, BioGenomics (Catalog #AAA653503) is a DNA Ladder and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CpG-B DNA, K-type, BioGenomics can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in biological assays in vitro to activate cells and as an immune modulating agent. Dilution: In vitro Stimulation: 0.05-3uM. Researchers should empirically determine the suitability of the CpG-B DNA, K-type, BioGenomics for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CpG-B DNA, K-type, BioGenomics, DNA Ladder" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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