Western Blot (WB)
(Western blot analysis of extracts from DU145 cells, using B7-H6 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human B7-H6 Polyclonal Antibody | anti-NCR3LG1 antibody
B7-H6 Antibody
Gene Names
NCR3LG1; B7H6; B7-H6; DKFZp686O24166
Reactivity
Human
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
B7-H6; Polyclonal Antibody; B7-H6 Antibody; B7 homolog 6; B7H6; B7H6_HUMAN; DKFZp686O24166; Natural cytotoxicity triggering receptor 3 ligand 1; NCR3LG1; Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167; anti-NCR3LG1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
B7-H6 Antibody detects endogenous levels of total B7-H6.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
454
Applicable Applications for anti-NCR3LG1 antibody
ELISA (EIA)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ICC: 1:100-1:500
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human B7-H6, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from DU145 cells, using B7-H6 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from DU145 cells, using B7-H6 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Human gallbladder, using B7-H6 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Human gallbladder, using B7-H6 Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612275 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612275 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612275 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612275 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Related Product Information for anti-NCR3LG1 antibody
Function: Triggers NCR3-dependent natural killer cell activation.
Subcellular Location: Cell membrane>Single-pass type I membrane protein.
Tissue Specificity: Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).
Subunit Structure: Monomer. Interacts specifically with NCR3, but not with other natural killer cell-activating receptors, including NCR1, NCR2 and KLRK1.
Similarity: The C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV.
Subcellular Location: Cell membrane>Single-pass type I membrane protein.
Tissue Specificity: Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).
Subunit Structure: Monomer. Interacts specifically with NCR3, but not with other natural killer cell-activating receptors, including NCR1, NCR2 and KLRK1.
Similarity: The C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)50kD.
Predicted Molecular Weight: (Calculated)51kDa.
Predicted Molecular Weight: (Calculated)51kDa.
NCBI Official Full Name
natural cytotoxicity triggering receptor 3 ligand 1
NCBI Official Synonym Full Names
natural killer cell cytotoxicity receptor 3 ligand 1
NCBI Official Symbol
NCR3LG1
NCBI Official Synonym Symbols
B7H6; B7-H6; DKFZp686O24166
NCBI Protein Information
natural cytotoxicity triggering receptor 3 ligand 1
UniProt Protein Name
Natural cytotoxicity triggering receptor 3 ligand 1
Protein Family
UniProt Gene Name
NCR3LG1
UniProt Synonym Gene Names
B7H6; B7-H6
NCBI Description
B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]
Uniprot Description
B7-H6: Triggers NCR3-dependent natural killer cell activation.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: plasma membrane
Biological Process: regulation of immune response
Research Articles on NCR3LG1
Similar Products
Product Notes
The NCR3LG1 ncr3lg1 (Catalog #AAA9612275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B7-H6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the NCR3LG1 ncr3lg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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