Chick Fibroblast Basal Medium MCDB 201 Culture Media
Chick Fibroblast Basal Medium MCDB 201 (Powder)
Purity
Cell Culture Grade
Synonyms
Chick Fibroblast Basal Medium MCDB 201; Chick Fibroblast Basal Medium MCDB 201 (Powder); Chick Fibroblast Basal Medium MCDB 201 culture media
Purity/Purification
Cell Culture Grade
Form/Format
White, homogenous, free flowing powder
Endotoxin Level
< 1 EU/ml
pH
5.7
Solubility
Pale yellow, clear, complete
Directions per Liter
Dissolve 17.7g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as mg/liter
Inorganic Salts:
Ammonium Metavanadate: 0.00001
Ammonium Molybdate * H2O: 0.000618
Calcium Chloride * 2H20: 294.0
Cupric Sulfate * 5H20: 0.00025
Ferrous Sulfate * 7H20: 1.668
Magnesium Sulfate: 180.57
Manganese Sulfate: 0.000075
Nickel Chloride * 6H2O: 0.0000012
Potassium Chloride: 223.65
Sodium Chloride: 7597.0
Sodium Metasilicate * 9H2O: 0.1420
Sodium Phosphate Dibasic * H2O: 70.99
Sodium Selenite: 0.00087
Zinc Sulfate * 7H2O: 0.02874
Amino Acids:
L-Alanine 8.90
L-Arginine HCl 63.20
L-Asparagine * H2O: 150
L-Aspartic Acid: 13.31
L-Cysteine * HCl * H2O: 35.13
L-Glutamic Acid: 14.71
L-Glutamine: 146.15
Glycine: 7.51
L-Histidine * HCl * H2O: 20.97
L-Isoleucine: 13.12
L-Leucine: 39.35
L-Lysine * HCl: 36.54
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 5.76
L-Serine: 31.53
L-Threonine: 35.74
L-Tryptophan: 6.13
L-Tyrosine * Na2 * 2H2O: 11.35
L-Valine: 35.13
Vitamins:
Biotin: 0.00730
Choline Chloride: 13.96
Vitamin B12: 0.136
Folinic Acid, Calcium: 0.00512
Myo-Inositol: 18.02
Nicotinamide: 6.11
D-Pantothenic Acid * Ca: 0.477
Pyridoxine * HCl: 0.0617
Riboflavin: 0.113
Thiamine * HCl: 0.337
Other:
Adenine HCl: 0.172
D-Glucose: 1441.0
HEPES: 7149.0
Linoleic Acid: 0.0841
Lipoic Acid: 0.00206
Phenol Red, Sodium: 1.242
Putrescine * 2HCl: 0.00016
Sodium Pyruvate: 55.00
Thymidine: 0.0727
Total: 17.7g/liter
Ammonium Metavanadate: 0.00001
Ammonium Molybdate * H2O: 0.000618
Calcium Chloride * 2H20: 294.0
Cupric Sulfate * 5H20: 0.00025
Ferrous Sulfate * 7H20: 1.668
Magnesium Sulfate: 180.57
Manganese Sulfate: 0.000075
Nickel Chloride * 6H2O: 0.0000012
Potassium Chloride: 223.65
Sodium Chloride: 7597.0
Sodium Metasilicate * 9H2O: 0.1420
Sodium Phosphate Dibasic * H2O: 70.99
Sodium Selenite: 0.00087
Zinc Sulfate * 7H2O: 0.02874
Amino Acids:
L-Alanine 8.90
L-Arginine HCl 63.20
L-Asparagine * H2O: 150
L-Aspartic Acid: 13.31
L-Cysteine * HCl * H2O: 35.13
L-Glutamic Acid: 14.71
L-Glutamine: 146.15
Glycine: 7.51
L-Histidine * HCl * H2O: 20.97
L-Isoleucine: 13.12
L-Leucine: 39.35
L-Lysine * HCl: 36.54
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 5.76
L-Serine: 31.53
L-Threonine: 35.74
L-Tryptophan: 6.13
L-Tyrosine * Na2 * 2H2O: 11.35
L-Valine: 35.13
Vitamins:
Biotin: 0.00730
Choline Chloride: 13.96
Vitamin B12: 0.136
Folinic Acid, Calcium: 0.00512
Myo-Inositol: 18.02
Nicotinamide: 6.11
D-Pantothenic Acid * Ca: 0.477
Pyridoxine * HCl: 0.0617
Riboflavin: 0.113
Thiamine * HCl: 0.337
Other:
Adenine HCl: 0.172
D-Glucose: 1441.0
HEPES: 7149.0
Linoleic Acid: 0.0841
Lipoic Acid: 0.00206
Phenol Red, Sodium: 1.242
Putrescine * 2HCl: 0.00016
Sodium Pyruvate: 55.00
Thymidine: 0.0727
Total: 17.7g/liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Chick Fibroblast Basal Medium MCDB 201 culture media
Basal Medium MCDB 201 is specifically formulated for the cultivation of chick fibroblasts in accordance with the formulation by McKeehan and Ham. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed). Also suitable for stem cell and CHO cell growth.
Product Categories/Family for Chick Fibroblast Basal Medium MCDB 201 culture media
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Product Notes
The Chick Fibroblast Basal Medium MCDB 201 (Catalog #AAA652663) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Chick Fibroblast Basal Medium MCDB 201, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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