Tumor Necrosis Factor alpha, Rat Active Protein | Tnf active protein

Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)

Cat. Num. AAA650716

• Gene Names: Tnf; Tnfa; RATTNF; TNF-alpha
• Purity: Highly Purified; 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
• Synonyms: Tumor Necrosis Factor alpha; Rat; Recombinant; Rat (TNFa); Tnf active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
• Form/Format: Supplied as a lyophilized powder from 0.5X PBS, pH 7.1 (0.2um filtered). Reconstitute in sterile dH2O, carrier protein to 0.1-1mg/ml. Do not vortex.
• Sequence: MLRSSSQNSS DKPVVHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL

• Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is 0.05ng/ml, corresponding to a specific activity of; 5x10e7 units/mg.
• Country of Origin: USA
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Tnf active protein

Tumor Necrosis Factor-alpha (TNF-alpha) is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF superfamily of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation and diabetes.

Recombinant rat TNF-alpha is a soluble 17.3kD protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein containing 157 amino acid residues.

Product Categories/Family for Tnf active protein

Growth Factors; Cytokines; Growth Factors-TNF

NCBI and Uniprot Product Information

• NCBI GI #: 6981662
• NCBI GeneID: 24835
• NCBI Accession #: NP_036807.1
• NCBI GenBank Nucleotide #: NM_012675.3
• UniProt Accession #: P16599; Q6EE11; Q9JI26; Q9JI27
• Molecular Weight: 17.3kD
• NCBI Official Full Name: tumor necrosis factor
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: Tnf
• NCBI Official Synonym Symbols: Tnfa; RATTNF; TNF-alpha
• NCBI Protein Information: tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2
• UniProt Protein Name: Tumor necrosis factor
• Protein Family: Tumor necrosis factor
• UniProt Gene Name: Tnf
• UniProt Synonym Gene Names: Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_RAT

NCBI Description

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

Uniprot Description

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells

By similarity.

Subunit structure: Homotrimer. Interacts with SPPL2B

By similarity.

Subcellular location: Cell membrane; Single-pass type II membrane protein

By similarity. Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein

By similarity. Tumor necrosis factor, soluble form: Secreted

By similarity. C-domain 1: Secreted

By similarity. C-domain 2: Secreted

By similarity.

Post-translational modification: The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space

By similarity.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1

By similarity.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid

By similarity.

Sequence similarities: Belongs to the tumor necrosis factor family.