Mouse Mediator of DNA Damage Checkpoint Protein 1 Monoclonal Antibody | anti-MDC1 antibody
Mediator of DNA Damage Checkpoint Protein 1 (MDC1)
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography.
Synonyms
Mediator of DNA Damage Checkpoint Protein 1; Monoclonal Antibody; Mediator of DNA Damage Checkpoint Protein 1 (MDC1); anti-MDC1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
P2B11
Specificity
Recognizes mouse MDC1 at ~184kD. Species Crossreactivity: Human, chimpanzee and bovine.
Purity/Purification
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-MDC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
Immunofluorescence (IC-IF): 1:100; Western Blot: 1:2000
Immunogen
GST-tagged recombinant protein corresponding to mouse MDC1 at N-terminal.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-MDC1 antibody
MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139.
References
1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781.
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Product Notes
The MDC1 (Catalog #AAA6506764) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mediator of DNA Damage Checkpoint Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Immunofluorescence (IC-IF): 1:100; Western Blot: 1:2000 . Researchers should empirically determine the suitability of the MDC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mediator of DNA Damage Checkpoint Protein 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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