Rabbit anti-Human, Mouse XIAP Polyclonal Antibody | anti-XIAP antibody

XIAP (API3, Baculoviral IAP repeat-containing protein 4, BIRC4, hILP, HILP, IAP3, IAP-like protein, ILP1, Inhibitor of apoptosis protein 3, MIHA, Xiap, X-linked IAP, X-linked inhibitor of apoptosis protein) (HRP)

Cat. Num. AAA6506272

• Gene Names: XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: XIAP; Polyclonal Antibody; XIAP (API3; Baculoviral IAP repeat-containing protein 4; BIRC4; hILP; HILP; IAP3; IAP-like protein; ILP1; Inhibitor of apoptosis protein 3; MIHA; Xiap; X-linked IAP; X-linked inhibitor of apoptosis protein) (HRP); anti-XIAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Specific for human X-chromosome linked inhibitor of apoptosis (XIAP). Detects a band of ~57kD in human kidney lysates. Species Crossreactivity: Mouse
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-XIAP antibody: Immunohistochemistry (IHC) Frozen/Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 2ug/ml, Requires antigen retrieval using heat treatment prior to staining of paraffin sections.; WB: 0.5-2.0ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a thirteen amino acid sequence with the C-terminal region of human XIAP.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Immunohistochemistry (IHC)

(XIAP-AHP937 staining of human kidney tissue with anti-XIAP at 2ug/ml. Formalin fixed paraffin processed tissue.)

Product Categories/Family for anti-XIAP antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 32528299
• NCBI GeneID: 331
• NCBI Accession #: NP_001158.2
• NCBI GenBank Nucleotide #: NM_001167.3
• UniProt Accession #: P98170; Q9NQ14; D3DTF2
• Molecular Weight: 56,685 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase XIAP
• NCBI Official Synonym Full Names: X-linked inhibitor of apoptosis
• NCBI Official Symbol: XIAP
• NCBI Official Synonym Symbols: API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
• NCBI Protein Information: E3 ubiquitin-protein ligase XIAP; X-linked IAP; IAP-like protein; inhibitor of apoptosis protein 3; baculoviral IAP repeat-containing protein 4
• UniProt Protein Name: E3 ubiquitin-protein ligase XIAP
• Protein Family: XIAP-associated factor
• UniProt Gene Name: XIAP
• UniProt Synonym Gene Names: API3; BIRC4; IAP3; ILP; hILP; IAP-3; hIAP-3; hIAP3
• UniProt Entry Name: XIAP_HUMAN

NCBI Description

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011]

Uniprot Description

XIAP: an apoptotic suppressor protein. Inhibist apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Inhibits cell-death proteases, caspase-3, caspase-7, and perhaps caspase-9.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Apoptosis

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: nucleoplasm; spindle microtubule; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: Wnt receptor signaling pathway; positive regulation of protein ubiquitination; apoptosis; regulation of inflammatory response; regulation of BMP signaling pathway; protein ubiquitination; negative regulation of caspase activity; response to DNA damage stimulus; copper ion homeostasis; regulation of innate immune response; negative regulation of apoptosis; regulation of cell proliferation

Disease: Lymphoproliferative Syndrome, X-linked, 1; Lymphoproliferative Syndrome, X-linked, 2