CD40 Ligand, soluble Active Protein

CD40 Ligand, soluble, Recombinant, Human (CD40 Ligand, CD40-L, CD40L, CD40LG, hCD40L, sCD40L, CD178, gp39, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Su

Cat. Num. AAA650421

• Purity: Molecular Biology Grade; ~98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
• Synonyms: CD40 Ligand; soluble; Recombinant; Human (CD40 Ligand; CD40-L; CD40L; CD40LG; hCD40L; sCD40L; CD178; gp39; HIGM1; IGM; IMD3; T-BAM; T-cell Antigen GP39; TNF-related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; TNF Ligand Su; soluble active protein

• Host: Human, recombinant (E Coli)
• Purity/Purification: Molecular Biology Grade; ~98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
• Form/Format: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5. Reconstitute with 5-10mM sodium phosphate, pH 7.5, to a concentration of 0.1-1mg/ml. Do not vortex. Further dilute with PBS, 0.1% BSA or HSA.
• Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

• Biological Activity: Assay 1: Determined by the stimulation of IL-12 induction by human PBMCs.; Assay 2: Determined by the does-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5-10ng/ml.; Note: Results may vary with PBMC donor.
• Country of Origin: USA
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile 5-10mM sodium phosphate, 0.1% BSA or HSA. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CD40 Ligand, soluble active protein

Recombinant human soluble CD40 ligand is a 16.3kD protein containing 149aa residues comprising the receptor binding TNF-like domain of CD40L.

CD40 is a member of the TNF receptor family. it is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to ?immunodeficiency with hyper-IgM, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261aa type II transmembrane protein, which contains a 22aa cytoplasmic domain, a 24aa transmembrane domain and a 215aa extracellular domain. The soluble form of CD40L is an 18kD protein comprising the entire TNF homologous region of CD40L. It is generated in vivo by an intracellular proteolytic processing of the full length CD40L.
CD40 Ligand (CD40L/TRAP, CD154) is a type II membrane protein. It effectuates the helper function of T cells on resting B cells. Recombinant human soluble CD40 Ligand (sCD40L/TRAP) is a 16.3kD protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
The CD40 antigen contains four homologous repeating cysteine-rich extracellular domains. It is a single chain glycoprotein. It is known to be a member of the tumor necrosis factor/nerve growth factor superfamily. It shows a significant homology to the Hodgkin's disease-associated antigen, CD30. CD40 is present on all B cells except plasma cells. It is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 is found on normal and neoplastic B cells, strongly expressed by interdigitating cells (IDC), basal epithelial cells, carcinomas, macrophages, some endothelial cells, and follicular dendritic cells. It is a pan-B marker, absent only from terminally differentiated plasma cells.
CD40 receptor is expressed on antigen-presenting cells such as B cells, macrophages, dendritic cells and thymic epithelial cells. CD40 mediates stimulation of cell growth, differentiation and death. It also plays a role in regulating transcription in B cells.
CD40 is predicted to function in the process of B cell selection in the germinal center. Studies demonstrated that it induces strong homotypic adhesions in resting B cells. Together with Interleukin-4 (IL-4) maintains the cell cycle of B blasts and promotes the switch to IgE secretion. Puri ed B cells cultured in the CD40 system in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. The ligand for CD40 is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion.

Product Categories/Family for CD40 Ligand, soluble active protein

Growth Factors; Cytokines; Growth Factors-TRAP

NCBI and Uniprot Product Information

• NCBI GI #: 114053977
• Molecular Weight: 16.3kD
• NCBI Official Full Name: CD40

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: signal transducer activity; protein binding; enzyme binding; ubiquitin protein ligase binding; receptor activity; antigen binding

Biological Process: B cell proliferation; platelet activation; positive regulation of isotype switching to IgG isotypes; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; protein kinase B signaling cascade; positive regulation of B cell proliferation; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; positive regulation of protein amino acid phosphorylation; inflammatory response; defense response to virus; regulation of immunoglobulin secretion

Disease: Immunodeficiency With Hyper-igm, Type 3