Epidermal Growth Factor, Pro- Active Protein | EGF active protein

Epidermal Growth Factor, Pro-, Recombinant, Mouse (Pro-EGF, EGF) (BSA Free)

Cat. Num. AAA650397

• Gene Names: EGF; URG; HOMG4
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Epidermal Growth Factor; Pro-; Recombinant; Mouse (Pro-EGF; EGF) (BSA Free); EGF active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured by its ability to stimulate proliferation of Balb/3T3, a mouse fibroblast cell line. The ED 50 for this effect is typically 1-5 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin).
• Endotoxin Level: < 1.0 EU per 1 g of the growth factor as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for EGF active protein

EGF is the prototypic member of a family of growth factors that also includes amphiregulin, betacellulin, epigen, epiregulin, HB-EGF, neuregulins-1 through -6, and TGF- (1). These proteins contain EGF-like domains with three intramolecular disulfide bonds between conserved cysteines (2). EGF family members are synthesized as transmembrane preproproteins with varying numbers of EGF-like domains (3). The extracellular region of mouse pro-EGF contains eight LDL R class B repeats and nine EGF-like domains (4). Mature EGF is derived from the juxtamembrane EGF-like domain. EGF binds ErbB1 and induces the formation of homodimers or heterodimers containing ErbB2 (5). pro-EGF is most highly expressed in the submaxillary gland and kidney (6). In the kidney, the 190 kD preproprotein is cleaved by membrane-associated serine proteases, liberating the extracellular region which is subsequently processed into smaller fragments including the 6 kD mature EGF (7-10). The various cleavage products produced in the kidney also are present in urine (9-11). In the submaxillary gland, however, nearly all EGF is processed intracellularly and stored in secretory vesicles (6, 12). The soluble precursor binds ErbB1 and induces cellular proliferation, although it is significantly less potent than mature EGF (8, 9). In human thyroid carcinoma cells, a splice variant of pro-EGF with a deletion in the cytoplasmic domain induces increased proliferative activity relative to wild type pro-EGF (13). Within the extracellular region, mouse pro-EGF shares 79% aa sequence identity with rat pro-EGF and 67%-69% with human, canine, feline, and porcine pro-EGF.

Product Categories/Family for EGF active protein

Growth Factors; Cytokines; Growth Factors-EGF

NCBI and Uniprot Product Information

• NCBI GI #: 46242544
• NCBI GeneID: 1950
• UniProt Accession #: Q6QBS2; P01133
• Molecular Weight: Based on the N-terminal sequencing, the recombinant mouse pro-EGF, generated by removal of its signal peptide, starts at Trp 29 and has a predicted molecular mass of 110 kD. As a result of glycosylation the recombinant mouse EGF migrates as a 160 kD prote
• NCBI Official Full Name: epidermal growth factor
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor; beta-urogastrone; OTTHUMP00000162922; OTTHUMP00000219721; OTTHUMP00000219722
• UniProt Protein Name: Epidermal growth factor
• Protein Family: Pro-epidermal growth factor
• UniProt Gene Name: EGF
• UniProt Entry Name: Q6QBS2_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.