Rabbit anti-Human, Mouse TPI1 Polyclonal Antibody | anti-TPI1 antibody

TPI1, NT (Triosephosphate Isomerase, TIM, Triose-phosphate Isomerase, TPI) (HRP)

Cat. Num. AAA6503602

• Gene Names: TPI1; TIM; TPI; TPID; HEL-S-49
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TPI1; Polyclonal Antibody; NT (Triosephosphate Isomerase; TIM; Triose-phosphate Isomerase; TPI) (HRP); anti-TPI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TPI1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TPI1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human TPI1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of TPI1 Antibody (N-term) in Y79(lane 1),CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml))

Western Blot (WB)

(Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TPI1 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TPI1 antibody

Antibodies; Enzymes; Isomerase

References

1. Martins-de-Souza, D., et.al., BMC Psychiatry 9, 17 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507645
• NCBI GeneID: 7167
• NCBI Accession #: NP_000356
• NCBI GenBank Nucleotide #: NM_000365.5
• UniProt Accession #: P60174; P00938; Q6FHP9; Q6IS07; Q8WWD0; Q96AG5; B7Z5D8; D3DUS9
• Molecular Weight: 28.8kDa (269aa) confirmed by MALDI-TOF
• NCBI Official Full Name: triosephosphate isomerase isoform 1
• NCBI Official Synonym Full Names: triosephosphate isomerase 1
• NCBI Official Symbol: TPI1
• NCBI Official Synonym Symbols: TIM; TPI; TPID; HEL-S-49
• NCBI Protein Information: triosephosphate isomerase
• UniProt Protein Name: Triosephosphate isomerase
• UniProt Gene Name: TPI1
• UniProt Synonym Gene Names: TPI; TIM
• UniProt Entry Name: TPIS_HUMAN

NCBI Description

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]