Interleukin 3 Active Protein | IL3 active protein

Interleukin 3, Recombinant, Mouse (IL-3)

Cat. Num. AAA650159

• Gene Names: IL3; IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
• Purity: Highly Purified; Highly Purified (98% by SDS-PAGE analysis).
• Synonyms: Interleukin 3; Recombinant; Mouse (IL-3); IL3 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; Highly Purified (98% by SDS-PAGE analysis).
• Form/Format: Supplied as a lyophilized protein without additives. Reconstitute with sterile dH2O to a concentration of 0.1-1.0mg/ml with carrier protein (0.1% HSA or BSA).
• Sequence: MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC

• Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is; 0.05ng/ml, corresponding to a specific activity of 2 x 10e7u/mg.
• Country of Origin: USA
• Endotoxin Level: 0.1ng/ug (1EU/ug).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O and carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IL3 active protein

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant murine IL-3 is a 15.1kD globular protein containing 135 amino acid residues.

Product Categories/Family for IL3 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 28416915
• NCBI GeneID: 3562
• NCBI Accession #: NP_000579.2
• NCBI GenBank Nucleotide #: NM_000588.3
• UniProt Accession #: P08700; Q6GS87; Q6NZ78; Q6NZ79
• Molecular Weight: 15.1kD
• NCBI Official Full Name: interleukin-3
• NCBI Official Synonym Full Names: interleukin 3 (colony-stimulating factor, multiple)
• NCBI Official Symbol: IL3
• NCBI Official Synonym Symbols: IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
• NCBI Protein Information: interleukin-3; OTTHUMP00000065963; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
• UniProt Protein Name: Interleukin-3
• Protein Family: Interleukin
• UniProt Gene Name: IL3
• UniProt Entry Name: IL3_HUMAN

NCBI Description

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq]

Uniprot Description

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Tissue specificity: Activated T-cells, mast cells, natural killer cells.

Sequence similarities: Belongs to the IL-3 family.