Typical Standard Curve/Testing Data
Rat Orexin receptor type 2 (HCRTR2) ELISA Kit | HCRTR2 elisa kit
Rat Orexin receptor type 2 (HCRTR2) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: HCRTR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HCRTR2 antibody and an HCRTR2-HRP conjugate. The assay sample and buffer are incubated together with HCRTR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HCRTR2 concentration since HCRTR2 from samples and HCRTR2-HRP conjugate compete for the anti-HCRTR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HCRTR2 from the sample, fewer sites are left to bind HCRTR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HCRTR2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A. [provided by RefSeq, Jan 2009]
Uniprot Description
HCRTR2: Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: 6p12
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: neuropeptide receptor activity; orexin receptor activity; peptide hormone binding; peptide binding
Biological Process: synaptic transmission; neuropeptide signaling pathway; circadian sleep/wake cycle process; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); cellular response to hormone stimulus; regulation of circadian sleep/wake cycle, sleep
Research Articles on HCRTR2
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Product Notes
The Rat HCRTR2 hcrtr2 (Catalog #AAA7223531) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7223531 ELISA Kit recognizes Rat HCRTR2. It is sometimes possible for the material contained within the vial of "Orexin receptor type 2 (HCRTR2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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