Rabbit anti-Human STK11 Polyclonal Antibody | anti-STK11 antibody

STK11, CT (Serine/Threonine-protein Kinase 11, Serine/Threonine-protein Kinase LKB1, Renal Carcinoma Antigen NY-REN-19, LKB1, PJS) (APC)

Cat. Num. AAA6501189

• Gene Names: STK11; PJS; LKB1; hLKB1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: STK11; Polyclonal Antibody; CT (Serine/Threonine-protein Kinase 11; Serine/Threonine-protein Kinase LKB1; Renal Carcinoma Antigen NY-REN-19; LKB1; PJS) (APC); anti-STK11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STK11.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-STK11 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human STK11 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-STK11 antibody

Antibodies; Protein Kinases

References

1. Mart, et al., J. Biotechnol. 115(1):23-34 (2005). 2. Shaw, R.J., et al., Proc. Natl. Acad. Sci. USA 101(10):3329-3335 (2004). 3. Suzuki, A., et al., Biochem. Biophys. Res. Commun. 324(3):986-992 (2004). 4. Hearle, N., et al., Genes Chromosomes Cancer 41(2):163-169 (2004). 5. Corradetti, M.N., et al., Genes Dev. 18(13):1533-1538 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4507271
• NCBI GeneID: 6794
• NCBI Accession #: NP_000446
• NCBI GenBank Nucleotide #: NM_000455.4
• UniProt Accession #: Q15831; B2RBX7; E7EW76
• Molecular Weight: 51.0kDa (456aa)
• NCBI Official Full Name: serine/threonine-protein kinase STK11
• NCBI Official Synonym Full Names: serine/threonine kinase 11
• NCBI Official Symbol: STK11
• NCBI Official Synonym Symbols: PJS; LKB1; hLKB1
• NCBI Protein Information: serine/threonine-protein kinase STK11
• UniProt Protein Name: Serine/threonine-protein kinase STK11
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK11
• UniProt Synonym Gene Names: LKB1; PJS; LKB1; hLKB1
• UniProt Entry Name: STK11_HUMAN

NCBI Description

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

LKB1: a S/T protein kinase of the CAMKL family. A tumor suppressor that helps control cell structure, polarity, apoptosis and energy homeostasis. Phosphorylates AGS3 (activator of G-protein signaling 3) GPR domains, regulating the interaction of GPR-containing proteins with G-proteins. A cytosolic protein complex comprised of LKB1, the pseudokinase STRAD, and the MO25 scaffold protein. Activates AMPK and several related protein kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. The Lkb1/Strad/Mo25 complex can polarize single epithelial cells, leading to the formation of a brush border containing microvilli on the apical surface. Mutation in the corresponding gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues.

Protein type: Protein kinase, CAMK; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Autophagy; Tumor suppressor; CAMK group; CAMKL family; LKB subfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; magnesium ion binding; protein complex binding; protein kinase activator activity; ATP binding; LRR domain binding

Biological Process: Golgi localization; response to glucagon stimulus; protein heterooligomerization; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; response to lipid; glucose homeostasis; T cell receptor signaling pathway; protein amino acid phosphorylation; anoikis; negative regulation of cell proliferation; positive regulation of gluconeogenesis; regulation of fatty acid biosynthetic process; vasculature development; tissue homeostasis; regulation of cell growth; cell cycle arrest; positive thymic T cell selection; regulation of Wnt receptor signaling pathway; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; axonogenesis; establishment of cell polarity; insulin receptor signaling pathway; activation of protein kinase activity; energy reserve metabolic process; autophagy; positive regulation of axonogenesis; negative regulation of cell growth; response to ionizing radiation; response to DNA damage stimulus; spermatid development

Disease: Pancreatic Cancer; Testicular Germ Cell Tumor; Peutz-jeghers Syndrome