Testing Data
(Data show performance of mouse TNF-alpha compared to a leading competitor's protein in L929 Cell Cytotoxic Assay.)
Tumor Necrosis Factor alpha Active Protein | tnfa active protein
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)
Purity
Highly Purified~95% (SDS-PAGE)
Synonyms
Tumor Necrosis Factor alpha; Recombinant; Mouse (TNFa); tnfa active protein
Purity/Purification
Highly Purified
~95% (SDS-PAGE)
~95% (SDS-PAGE)
Form/Format
Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.
Biological Activity
ED50 range=0.005-0.02ng/ml, determined by the dose dependent cytotoxicity of L929 cells. Optimal concentration for individual application should be determined by a dose response assay.
Endotoxin Level
~0.1ng/ug
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Data show performance of mouse TNF-alpha compared to a leading competitor's protein in L929 Cell Cytotoxic Assay.)
Testing Data
(Data show performance of mouse TNF-alpha compared to a leading competitor's protein in L929 Cell Cytotoxic Assay.)
Related Product Information for tnfa active protein
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Product Categories/Family for tnfa active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.5kD
NCBI Official Full Name
tumor necrosis factor alpha
NCBI Official Symbol
tnfa
NCBI Protein Information
tumor necrosis factor alpha
Protein Family
Research Articles on tnfa
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Product Notes
The tnfa (Catalog #AAA650048) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor alpha, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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