Rabbit RSK1 Polyclonal Antibody | anti-RSK1 antibody

RSK1 (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Protein S6 Kinase alpha-1, RPS

Cat. Num. AAA6498221

• Gene Names: RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: RSK1; Polyclonal Antibody; RSK1 (Ribosomal S6 Kinase 1; RSK-1; 90kD Ribosomal Protein S6 Kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP Kinase-activated Protein Kinase 1a; MAPK-activated Protein Kinase 1a; MAPKAP Kinase 1a; MAPKAPK1A; MAPKAPK-1a; Ribosomal Protein S6 Kinase alpha-1; RPS; Ribosomal Protein S6 Kinase 90kDa Polypeptide 1; EC=2.7.11.1; HU-1; anti-RSK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human p90 RSK1. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RSK1 antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to the CT sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: NIH/3T3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-RSK1 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein RSK1 (~90kD).)

Western Blot (WB)

(Western Blot Analysis:NIH/3T3 lysate (Lane 1), Huvec lysate (Lane 2), A431 lysate (Lane 3) and HepG2 lysate (Lane 4) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-RSK1 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein RSK1 (~83kD).)

Product Categories/Family for anti-RSK1 antibody

Antibodies; Protein Kinases; Ribosomal

NCBI and Uniprot Product Information

• NCBI GI #: 55743134
• NCBI GeneID: 6195
• NCBI Accession #: NP_001006666.1
• NCBI GenBank Nucleotide #: NM_001006665.1
• UniProt Accession #: Q15418; Q5SVM5; Q5SVM8; Q5SVM9; Q96C05; Q9BQK2; A6NGG4; A8K9K7; B2RDY8; B7Z5J0
• Molecular Weight: 82,723 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-1 isoform b
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• NCBI Official Symbol: RPS6KA1
• NCBI Official Synonym Symbols: RSK; HU-1; RSK1; MAPKAPK1A
• NCBI Protein Information: ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinas
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-1
• UniProt Gene Name: RPS6KA1
• UniProt Synonym Gene Names: MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
• UniProt Entry Name: KS6A1_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Translation; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; magnesium ion binding; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of translation in response to stress; negative regulation of caspase activity; toll-like receptor 3 signaling pathway; signal transduction; cell cycle; positive regulation of cell growth; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis