Rabbit anti-Human, Mouse PTPN3 Polyclonal Antibody | anti-PTPN3 antibody

PTPN3 (Protein Tyrosine Phosphatase Non-receptor Type 3, Protein Tyrosine Phosphatase H1, PTP-H1, PTPH1) (APC)

Cat. Num. AAA6495720

• Gene Names: PTPN3; PTPH1; PTP-H1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PTPN3; Polyclonal Antibody; PTPN3 (Protein Tyrosine Phosphatase Non-receptor Type 3; Protein Tyrosine Phosphatase H1; PTP-H1; PTPH1) (APC); anti-PTPN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPN3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PTPN3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PTPN3 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PTPH1 Center Pab is used in Western blot to detect PTPH1 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PTPH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PTPN3 antibody

Antibodies; Enzymes; Phosphatase

References

1. Ikuta, S., et al., J. Gastroenterol. 29(6):727-732 (1994). 2. Arimura, Y., et al., Tumour Biol. 13(3):180-186 (1992). 3. Yang, Q., et al., Proc. Natl. Acad. Sci. USA 88(14):5949-5953 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 223941879
• NCBI GeneID: 5774
• NCBI Accession #: NP_001138840.1
• NCBI GenBank Nucleotide #: NM_001145368.1
• UniProt Accession #: P26045; A0AUW9; E7EN99; E9PGU7
• Molecular Weight: 83,723 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 3 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 3
• NCBI Official Symbol: PTPN3
• NCBI Official Synonym Symbols: PTPH1; PTP-H1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 3
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 3
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN3
• UniProt Synonym Gene Names: PTPH1; PTP-H1

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity.