Rabbit CRAT Polyclonal Antibody | anti-CRAT antibody

CRAT (Carnitine O-acetyltransferase, Carnitine Acetylase, Carnitine Acetyltransferase, CrAT, CAT, CAT1)

Cat. Num. AAA649535

• Gene Names: CRAT; CAT1
• Applications: ELISA, Western Blot
• Purity: Highly Purified; Purified by ammonium sulfate precipitation and Ion Exchange chromatography.
• Synonyms: CRAT; Polyclonal Antibody; CRAT (Carnitine O-acetyltransferase; Carnitine Acetylase; Carnitine Acetyltransferase; CrAT; CAT; CAT1); Anti -CRAT (Carnitine O-acetyltransferase; anti-CRAT antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Carnitine Acetyltransferase.
• Purity/Purification: Highly Purified; Purified by ammonium sulfate precipitation and Ion Exchange chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

• Applicable Applications for anti-CRAT antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunoelectrophoresis, Single Radial Immunodiffusion and Inhibition.
• Immunogen: Carnitine acetyltransferase isolated and purified from pigeon breast muscle
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-CRAT antibody

Antibodies; Abs to Enzymes; Acetyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 21618331
• NCBI GeneID: 1384
• NCBI Accession #: NP_000746.2
• NCBI GenBank Nucleotide #: NM_000755.3
• UniProt Accession #: P43155; Q5T952; Q9BW16
• Molecular Weight: 70,858 Da
• NCBI Official Full Name: carnitine O-acetyltransferase isoform 1
• NCBI Official Synonym Full Names: carnitine O-acetyltransferase
• NCBI Official Symbol: CRAT
• NCBI Official Synonym Symbols: CAT1
• NCBI Protein Information: carnitine O-acetyltransferase; CAT; carnitine acetylase; carnitine acetyltransferase
• UniProt Protein Name: Carnitine O-acetyltransferase
• Protein Family: Carnitine O-acetyltransferase
• UniProt Gene Name: CRAT
• UniProt Synonym Gene Names: CAT1; Carnitine acetylase; CAT; CrAT
• UniProt Entry Name: CACP_HUMAN

NCBI Description

This gene encodes carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Function: Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.

Catalytic activity: Acetyl-CoA + carnitine = CoA + O-acetylcarnitine.

Subunit structure: Monomer. Ref.7

Subcellular location: Endoplasmic reticulum

Potential. Peroxisome

Potential. Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Potential. Isoform 1: Mitochondrion

Potential. Isoform 2: Peroxisome

Potential.

Tissue specificity: Mostly in skeletal muscle, less in heart, liver and pancreas, only weakly detectable in brain, placenta, lung and kidney.

Sequence similarities: Belongs to the carnitine/choline acetyltransferase family.