Typical Testing Data/Standard Curve (for reference only)
Human Carnitine O acetyltransferase/Carnitine acetylase (C/CAT1) ELISA Kit | C elisa kit
Human Carnitine O acetyltransferase/Carnitine acetylase (C/CAT1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CAT antibody and an CAT-HRP conjugate. The assay sample and buffer are incubated together with CAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAT concentration since CAT from samples and CAT-HRP conjugate compete for the anti-CAT antibody binding site. Since the number of sites is limited, as more sites are occupied by CAT from the sample, fewer sites are left to bind CAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Uniprot Description
CRAT: Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. Belongs to the carnitine/choline acetyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Acetyltransferase; EC 2.3.1.7; Mitochondrial
Chromosomal Location of Human Ortholog: 9q34.1
Cellular Component: peroxisomal matrix; mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; peroxisome
Molecular Function: carnitine O-acetyltransferase activity; receptor binding
Biological Process: carnitine metabolic process, CoA-linked; fatty acid beta-oxidation using acyl-CoA oxidase; transport; cellular lipid metabolic process
Research Articles on C
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Product Notes
The Human C crat (Catalog #AAA7233272) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7233272 ELISA Kit recognizes Human C. It is sometimes possible for the material contained within the vial of "Carnitine O acetyltransferase/Carnitine acetylase (C/CAT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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