Rabbit anti-Human Protein Kinase D2 Polyclonal Antibody | anti-PKD2 antibody

Protein Kinase D2, Phosphorylated (Ser876) (PKD2) (MaxLight 750)

Cat. Num. AAA6495277

• Gene Names: PRKD2; PKD2; HSPC187; nPKC-D2
• Reactivity: Human
• Applications: Immunoblot, Western Blot, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Protein Kinase D2; Polyclonal Antibody; Phosphorylated (Ser876) (PKD2) (MaxLight 750); anti-PKD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human phospho-PKD2 (Ser876), Mr ~105kD. Two nonspecific proteins were also detected, one at ~120 kD and another at ~50 kD. The nonspecific bands were not competed by the immunizing peptide, and thus can serve as a loading control.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-PKD2 antibody: Immunoblot (IB), Western Blot (WB)
• Application Notes: IB: 0.5-2ug/ml of this lot detected phospho-PKD2 (Ser876) in RIPA lysates from PDBu (Phorbol 12,13-dibutyrate) stimulated HEK293 cells.; Applications are based on unconjugated antibody.
• Immunogen: Snthetic peptide corresponding to amino acids surrounding phosphorylated Serine 876 of human PKD2.
• Conjugate: MaxLight750
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Unstimlated (lane 1) and PDBu stimulated (lane 2) HEK293 cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho-PKD2 (Ser876) (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKD2 (Ser876) (105kD).)

Product Categories/Family for anti-PKD2 antibody

Antibodies; Protein Kinases

References

1. Sturany, S., et al., J. Biol. Chem. 276: 3310-3318, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 120659782
• NCBI GeneID: 25865
• NCBI Accession #: NP_001073349.1
• NCBI GenBank Nucleotide #: NM_016457
• UniProt Accession #: Q9BZL6; Q8TB08; Q9P0T6; Q9Y3X8
• Molecular Weight: 96,750 Da
• NCBI Official Full Name: serine/threonine-protein kinase D2 isoform A
• NCBI Official Synonym Full Names: protein kinase D2
• NCBI Official Symbol: PRKD2
• NCBI Official Synonym Symbols: PKD2; HSPC187; nPKC-D2
• NCBI Protein Information: serine/threonine-protein kinase D2
• UniProt Protein Name: Serine/threonine-protein kinase D2
• Protein Family: Polycystin
• UniProt Gene Name: PRKD2
• UniProt Synonym Gene Names: PKD2
• UniProt Entry Name: KPCD2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PRKD2: a calcium-independent, phospholipid-dependent CAMK kinase of the PKD family. Activated by diacylglycerol and phorbol esters. may play a role in carcinoid tumor progression.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Motility/polarity/chemotaxis; Kinase, protein; CAMK group; PKD family

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: cell death; positive regulation of cell adhesion; protein amino acid autophosphorylation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of fibroblast growth factor receptor signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; T cell receptor signaling pathway; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of angiogenesis; peptidyl-serine phosphorylation; positive regulation of T cell receptor signaling pathway; positive regulation of interleukin-2 production; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell adhesion