Rabbit Protein Kinase C, epsilon Polyclonal Antibody | anti-PKCe antibody

Protein Kinase C, epsilon (PKCe) (PE)

Cat. Num. AAA6495168

• Reactivity: Mouse, Human, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Protein Kinase C; epsilon; Polyclonal Antibody; epsilon (PKCe) (PE); anti-PKCe antibody

• Host: Rabbit
• Reactivity: Mouse, Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse PKCe, Mr 95kD. Species Crossreactivity: human and rat.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PKCe antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.; WB: 0.5-2ug/ml detects PKCe in Jurkat cell lysate. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunogen: Synthetic peptide, aa726-737 (KGFSYFGEDLMP), corresponding to the C-terminal variable (V5) region of mouse PKCe (KLH). Species sequence homology: rabbit.
• Conjugate: PE
• Included Positive Antigen Control: MBS656998
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis MBS656998 Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P9103-20C (0.5ug/ml). Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PKCe (~95kD).)

Product Categories/Family for anti-PKCe antibody

Antibodies; Protein Kinases

References

Blois, J. T. et al., (2004). J. Biol. Chem. 279:30123-30132. 1. Mischak, H., et al., J. Biol. Chem. 268: 6, 090-6, 096 (1993). 2. Borner, C., et al., J. Biol. Chem. 267: 12, 892-12, 899 (1992). 3. Wetsel, W.C., et al., J. Cell Biol. 117: 121-133 (1992). 4. Hug, H., et al., Biochem. J. 291: 329-343 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 6755084
• NCBI GeneID: 18754
• NCBI Accession #: NP_035234.1
• NCBI GenBank Nucleotide #: NM_011104.3
• UniProt Accession #: P16054
• Molecular Weight: 83,561 Da
• NCBI Official Full Name: protein kinase C epsilon type
• UniProt Protein Name: Protein kinase C epsilon type
• UniProt Gene Name: Prkce
• UniProt Synonym Gene Names: Pkce; Pkcea
• UniProt Entry Name: KPCE_MOUSE

Uniprot Description

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.

Protein type: EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; PKC family; Eta subfamily

Cellular Component: cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum; Golgi apparatus; Golgi membrane; membrane; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: actin monomer binding; ATP binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein kinase C activity; protein serine/threonine kinase activity; receptor activator activity; receptor binding; SH3 domain binding; transferase activity

Biological Process: cell adhesion; cell cycle; cell division; chemosensory behavior; immune system process; lipopolysaccharide-mediated signaling pathway; macrophage activation during immune response; negative regulation of apoptosis; negative regulation of protein ubiquitination; negative regulation of release of sequestered calcium ion into cytosol; peptidyl-serine phosphorylation; phosphorylation; positive regulation of actin filament polymerization; positive regulation of cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of insulin secretion; positive regulation of lipid catabolic process; positive regulation of MAPKKK cascade; positive regulation of synaptic transmission, GABAergic; protein amino acid phosphorylation; reduction of mitochondrial calcium ion concentration; regulation of lipid metabolic process; regulation of peptidyl-tyrosine phosphorylation; regulation of release of sequestered calcium ion into cytosol; release of sequestered calcium ion into cytosol; response to morphine