Rabbit anti-Human PSMB9 Polyclonal Antibody | anti-PSMB9 antibody

PSMB9, CT (Proteasome Subunit beta Type-9, Proteasome Subunit beta-1i, Proteasome Chain 7, Macropain Chain 7, Multicatalytic Endopeptidase Complex Chain 7, Really Interesting New Gene 12 Protein, RING12 Protein, Low Molecular Mass Protein 2, RING12, LMP2)

Cat. Num. AAA6494966

• Gene Names: PSMB9; LMP2; PSMB6i; RING12; beta1i
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PSMB9; Polyclonal Antibody; CT (Proteasome Subunit beta Type-9; Proteasome Subunit beta-1i; Proteasome Chain 7; Macropain Chain 7; Multicatalytic Endopeptidase Complex Chain 7; Really Interesting New Gene 12 Protein; RING12 Protein; Low Molecular Mass Protein 2; RING12; LMP2); anti-PSMB9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMB9.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PSMB9 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 193-219 in the C-terminal region of human PSMB9.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HL-60 cell line lysates (35ug/lane) usingMBS624924. PSMB9 (arrow) was detected usingMBS624924.)

Testing Data

(All lanes: MBS624924 (1:1000) Lane 1: A431 whole cell lysate Lane 2: Raji whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 23kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human prostate carcinoma usingMBS624924, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of HL60 cells using MBS624924 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PSMB9 antibody

Antibodies; Proteasomes

References

1. Honcharov, S.V., et.al., Fiziol Zh 55(2), 3-10 (2009). 2. Moschonas, A., et.al., Mol. Cell. Biol. 28(20), 6208-6222 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4506205
• NCBI GeneID: 5698
• NCBI Accession #: NP_002791.1
• NCBI GenBank Nucleotide #: NM_002800.4
• UniProt Accession #: P28065; Q16523; Q5JNW4; B0V0T1
• NCBI Official Full Name: proteasome subunit beta type-9 proprotein
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 9
• NCBI Official Symbol: PSMB9
• NCBI Official Synonym Symbols: LMP2; PSMB6i; RING12; beta1i
• NCBI Protein Information: proteasome subunit beta type-9; large multifunctional peptidase 2; low molecular mass protein 2; macropain chain 7; multicatalytic endopeptidase complex chain 7; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2); pr
• UniProt Protein Name: Proteasome subunit beta type-9
• Protein Family: Proteasome
• UniProt Gene Name: PSMB9
• UniProt Synonym Gene Names: LMP2; PSMB6i; RING12
• UniProt Entry Name: PSB9_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]

Uniprot Description

PSMB9: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.25.1; Nuclear receptor co-regulator; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle